FEMALE REPRODUCTIVE ORGANS AND THEIR INNERVATION

女性生殖器官及其神经支配

• 批准号: 6393379
• 负责人: RAYMOND E PAPKA
• 金额: $ 18.81万
• 依托单位: NORTHEAST OHIO MEDICAL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-09-15 至 2003-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6393379
• 关键词: birth; cervix; estrogen receptors; estrogens; immunocytochemistry; in situ hybridization; innervation; laboratory rat; neuroanatomy; neuroendocrine system; neurosurgery; neurotransmitters; pregnancy; uterus

DESCRIPTION: Long termgoals of this research seek tounderstand the morphological and functional substrates of the uterine innervation, the response of uterine nerves to steroid sex hormones and how these may play a role in such conditions as preterm labor, protracted labor, autonomic dysreflexia and pregnancy-induced hypertension. The current proposal investigates the autonomic and sensory neural substrate for integration of uterine information and relates these substrates to the altered physical & steroid hormone environment of pregnancy and parturition. Distinct subpopulations of autonomic and sensory neurons innervate the uterus. This specificity of neural organization led to the hypothesis: first, that uterine neurons in autonomic and sensory ganglia provide part of the substrate, which involves estrogen-receptive neurons, to integrate uterine information and secondly, that this substrate plays a role in pregnancy, parturition and viscerovisceral reflexes. A specific role for estrogen in these pathways is hypothesized because estrogen modulates neurochemical systems in the CNS and influences sensitivity of primary afferent nerves. Specific aims will: 1) characterize sympathetic and vagal inputs to the uterus and determine if autonomic and sensory ganglionic uterine-related neurons contain estrogen receptors (ER), 2) determine if the content and distribution of ER or ER-containing, uterine-related autonomic and sensory neurons change with the altering hormonal milieu of pregnancy & parturition, 3) characterize neurotransmitters of ER-containing neurons in autonomic and sensory ganglia, 4) evaluate whether the input activity to autonomic uterine-related neurons increases during pregnancy and parturition and 5) determine whether some uterine-related neurons influence the cervical ripening of parturition through release of transmitters that act in neurogenic inflammation. These studies will utilize retrograde tracing, in situ hybridization histochemistry, immunohistochemistry, nerve transections, parturition-induced activation of ganglionic neurons, and radioligand binding for ER. Health benefits from understanding involvement of neural mechanisms in the uterus include an increased basic understanding of neuroendocrine coordination of gestational events including pregnancy and parturition and the possibility of remediating problems such as preterm labor, protracted labor, autonomic dysreflexia and pregnancy-induced hypertension.

描述：本研究的长期目标旨在了解 子宫神经支配的形态和功能基质 子宫神经对类固醇性激素的反应以及它们如何发挥作用 在早产、旷产、自主分娩等情况下的作用 反射障碍和妊娠高血压。 目前的提案 研究自主神经和感觉神经基质的整合 子宫信息并将这些底物与改变的身体和 妊娠和分娩的类固醇激素环境。 清楚的 自主神经元和感觉神经元亚群支配子宫。 这 神经组织的特殊性导致了这样的假设：首先， 自主神经节和感觉神经节中的子宫神经元提供了部分 底物，涉及雌激素受体神经元，整合子宫 信息，其次，这种底物在怀孕中发挥作用， 分娩和内脏反射。 雌激素的特殊作用 假设这些途径是因为雌激素调节神经化学物质 中枢神经系统中的系统并影响初级传入神经的敏感性。 具体目标将：1）表征交感神经和迷走神经输入 子宫并确定自主神经和感觉神经节是否与子宫相关 神经元含有雌激素受体（ER），2）确定其含量和 ER 或含有 ER 的子宫相关自主神经和感觉的分布 神经元随着怀孕和分娩荷尔蒙环境的变化而变化， 3）表征自主神经和内质网神经元的神经递质 感觉神经节，4）评估输入活动是否自主神经 子宫相关神经元在怀孕和分娩期间增加，5) 确定一些与子宫相关的神经元是否影响宫颈 通过释放起作用的递质来促进分娩的成熟 神经源性炎症。 这些研究将利用逆行追踪， 原位杂交组织化学、免疫组织化学、神经横断、 分娩诱导的神经节神经元和放射性配体的激活 ER 的绑定。 了解神经机制的参与对健康有益 子宫包括增加对神经内分泌的基本了解 妊娠事件的协调，包括怀孕和分娩以及 纠正早产、旷产等问题的可能性 分娩、自主神经反射异常和妊娠高血压。