FEMALE REPRODUCTIVE ORGANS AND THEIR INNERVATION

女性生殖器官及其神经支配

• 批准号: 3405043
• 负责人: RAYMOND E PAPKA
• 金额: $ 10.54万
• 依托单位: UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-09-15 至 1993-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3405043
• 关键词: acetylcholine; afferent nerve; antibody; calcitonin gene related peptide; cervix; efferent nerve; electron microscopy; female; female reproductive system; ganglions; immunocytochemistry; immunofluorescence technique; innervation; laboratory rat; lectin; motor neurons; neuroanatomy; neurochemistry; neuroendocrine system; neuropeptide Y; neuropeptides; norepinephrine; parasympathetic nervous system; phase contrast microscopy; reproductive hormone; substance K; substance P; sympathetic nervous system; synapsins; uterus; vasoactive intestinal peptide

Hormones play a preeminent role in control of female reproductive processes; however, accumulating evidence indicates neural mechanisms, including afferent, efferent and central connections, are involved in reproductive processes, e.g. neuroendocrine reflexes, blood flow, smooth muscle control, sex steroid secretion, and parturition. Denervation of reproductive organs produces dysfunctions such as disrupted estrous cycles, decreased sex steroid secretion, implantation failure and prolonged gestation. Explanations of such problems are hampered by lack of fundamental information about the neurochemical anatomy of vascular, smooth muscular and glandular control and sensory reflexes in reproductive organs, e.g., the neurochemical anatomy of local sensory reflexes in reproductive organs, e.g., the neurochemical anatomy of local autonomic ganglia in pathways to genital organs is not well understood. Paracervical ganglia (PG) provide parasympathetic innervation to reproductive organs. Collaterals of primary sensory nerves ramify among PG neurons so these neurons could be influences not only by preganglionic nerves, but by sensory nerves as well. It is hypothesized that collaterals of peptide- containing sensory nerve fibers can interact with, and influence specific components of the female reproductive organs. Aims of this proposal are to extend current studies of the chemical neuroanatomy of the PG and their innervation of reproductive organs by examining sensory nerve- parasympathetic interactions in the PG. Using immunocytochemistry, double labeling, electron microscopy and axonal tracing methods we propose to address three specific objectives: #1 characterize in the PG the presence, distribution and source of and colocalization patterns of peptides in chemically-coded sensory nerves immunoreactive for substance P, neurokinin A, calcitonin gene-related peptide and galanin; #2 examine synaptic characteristics of the interactions of the peptidergic sensory nerves with PG neurons; #3 determine any patterns of association between these chemically-coded sensory nerves and PG neurons chemically-coded for NPT, VIP, acetylcholine and noradrenaline. This study will provide data to help design future experiments examining functional influences of nerves on reproductive processes, elucidating effects of hormones on the peripheral neurons innervating reproductive organs and defining functional components mediating neural-endocrine- reproductive end-organ reflexes.

激素在控制女性生殖方面发挥着重要作用 流程；然而，越来越多的证据表明神经机制， 包括传入、传出和中枢连接，参与 生殖过程，例如神经内分泌反射，血流顺畅 肌肉控制、性类固醇分泌和分娩。 去神经支配 生殖器官产生功能障碍，例如动情周期中断， 性类固醇分泌减少、着床失败和时间延长 妊娠。 对此类问题的解释因缺乏 有关血管、平滑肌的神经化学解剖学的基本信息 生殖器官的肌肉和腺体控制以及感觉反射， 例如，生殖局部感觉反射的神经化学解剖学 器官，例如局部自主神经节的神经化学解剖结构 通往生殖器官的途径尚不清楚。 宫颈旁神经节 (PG) 为生殖器官提供副交感神经支配。 初级感觉神经的侧支在 PG 神经元中分支，因此这些 神经元不仅受到节前神经的影响，还受到节前神经的影响 感觉神经也是如此。 据推测，肽的抵押物 含有感觉神经纤维可以与特定的相互作用并影响 女性生殖器官的组成部分。 该提案的目的是 扩展了 PG 及其化学神经解剖学的当前研究 通过检查感觉神经来观察生殖器官的神经支配 PG 中的副交感神经相互作用。 使用免疫细胞化学，双 我们建议使用标记、电子显微镜和轴突追踪方法 解决三个具体目标：#1 描述 PG 的存在， 肽的分布、来源和共定位模式 化学编码的感觉神经对 P 物质、神经激肽具有免疫反应 A、降钙素基因相关肽和甘丙肽； #2 检查突触 肽能感觉神经相互作用的特点 PG神经元； #3 确定这些之间的任何关联模式 化学编码的感觉神经和化学编码为 NPT 的 PG 神经元， VIP、乙酰胆碱和去甲肾上腺素。 这项研究将提供数据来帮助设计未来的实验 神经对生殖过程的功能影响，阐明 激素对支配生殖的周围神经元的影响 器官和定义介导神经内分泌的功能成分 生殖终末器官反射。