EXTRACELLULAR MATRIX AND RETINAL DEVELOPMENT

细胞外基质和视网膜发育

• 批准号: 6350871
• 负责人: Dale Hunter
• 金额: $ 25.04万
• 依托单位: TUFTS UNIVERSITY BOSTON
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-02-01 至 2002-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6350871
• 关键词: animal tissue; cell differentiation; cell migration; developmental neurobiology; extracellular matrix; immunocytochemistry; immunoprecipitation; in situ hybridization; laboratory mouse; laboratory rat; laminin; neurogenesis; neurons; nucleic acid probes; polymerase chain reaction; protein structure function; retina; tissue /cell culture

DESCRIPTION (Adapted from applicant's abstract): The development of the nervous system is modulated by both genetic and environmental phenomena. Whereas intrinsic factors undoubtedly play roles in the potential of progenitor cells to differentiate into a given cell type, extrinsic clues are likely to serve as signals for the timing of these differentiative events. The proposed project is designed to examine the effects of environmental factors on a cell's phenotype, particularly the environmental factors present in the extracellular matrix (ECM). Experiments are proposed to identify the distribution, expression, and function of components of the extracellular matrix, in particular, the beta2-containing laminins, during the development of the retina using antibodies and cDNA probes, both in vitro and in vivo. Together, it is anticipated that these lines of investigation should help to define the role of these extracellular matrix molecules in the development of the nervous system.

描述（根据申请人的摘要改编）： 神经系统受遗传和环境现象的调节。 而内在因素无疑在潜力中发挥了作用 祖细胞分化为给定的细胞类型，外部线索 很可能是这些分歧时机的信号 事件。 拟议的项目旨在检查 细胞表型的环境因素，尤其是环境 细胞外基质（ECM）中存在的因素。 提出了实验 确定组件的分布，表达和功能 特别是细胞外基质，含有β2的层粘连蛋白 使用抗体和cDNA探针的视网膜开发，都在 体内和体内。 共同预计 调查应有助于定义这些细胞外基质的作用 神经系统发展中的分子。