CORE--NEUROBEHAVIORAL FACILITY

核心--神经行为设施

• 批准号: 6302754
• 负责人: Randy J. Nelson
• 金额: $ 15.49万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-12-01 至 2000-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6302754
• 关键词: artificial respiration; behavioral /social science research tag; biomedical facility; cardiopulmonary resuscitation; cerebral ischemia /hypoxia; heart arrest; psychobiology; stroke

The general objective of this Core is to provide a facility behavioral effects of genetic, pharmacological, and surgical manipulations in mice and rats tat are recovering from stroke or cardiac arrest/CPR. Behavioral examination is an integral part of the post-ischemic recovery period and is correlated with physiological data and histological end-points for each animal. This is a novel approach in the field of experimental cerebral ischemia and cardiac arrest/CPR because long term behavioral outcomes are rarely examined. Most work has emphasized histological and morphological measures of injury (or recovery). Further, in some cases, behavioral changes may be the most salient phenotypic expression observed among mutant mice. In Aims 1 and 2, the behavioral consequences of sigma- receptor activation are assessed in normal and post-ischemic rats and in PPBP treated mice, as well as mice genetically deficient in neuronal nitric oxide synthase. In Aims 3 and 4, we will determine if neurobehavioral outcomes after experimental stroke are more favorable in female versus male rats and if stroke injury is exacerbated in transgenic mice deficient in estrogen receptors (estrogen receptor knock-outs). Lastly, cognitive dysfunction after cardiac arrest/CPR is evaluated in Aim 5 and 3 transgenic mouse strains as a function of neuronal cytotoxicity. The Neurobehavioral Core will utilize the animals obtained from each project to gain new insight into the functional vulnerability of specific brain regions to ischemic injury and the potential for plasticity in recovery of sensory motor function, memory, and simple cognitive tasks.

该核心的一般目标是提供设施的行为 遗传，药理和手术操纵对小鼠的影响 大鼠TAT正在从中风或心脏骤停/CPR中恢复。行为 检查是缺血后恢复期不可或缺的一部分， 与每个的生理数据和组织学终点相关 动物。这是实验大脑领域的一种新方法 缺血和心脏骤停/CPR，因为长期行为结果是 很少检查。大多数工作都强调了组织学和形态学 伤害（或恢复）的度量。此外，在某些情况下，行为 变化可能是观察到的最显着的表型表达 突变小鼠。在目标1和2中，Sigma-的行为后果 在正常和缺血后大鼠中评估受体激活 PPBP治疗的小鼠以及遗传缺乏神经元的小鼠 一氧化氮合酶。在目标3和4中，我们将确定是否 实验中风后的神经行为结果在 雌性大鼠与雄性大鼠，如果中风损伤在转基因中加剧 缺乏雌激素受体（雌激素受体敲除）的小鼠。 最后，在AIM中评估心脏骤停/CPR后的认知功能障碍 5和3转基因小鼠菌株与神经元细胞毒性的关系。 神经行为核心将利用从每种动物中获得的动物 项目以获得对特定功能脆弱性的新见解 大脑区域缺血性损伤和可塑性的潜力 感觉运动功能，内存和简单认知任务的恢复。