WOUND HEALING IN THE CORNEAL STROMA

角膜基质的伤口愈合

• 批准号: 6398997
• 负责人: DAVID M MAURICE
• 金额: $ 22.94万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-02-01 至 2004-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6398997
• 关键词: apoptosis; cell biology; cell cycle; cell death; cell migration; cell type; corneal epithelium; corneal stroma; digital imaging; electron microscopy; eye fundus photography; eye injury; fibroblasts; fluorescence microscopy; genetically modified animals; green fluorescent proteins; immunocytochemistry; iontophoresis therapy; laboratory mouse; leukocyte activation /transformation; microelectrodes; phagocytosis; wound healing

DESCRIPTION (provided by applicant): The movement and morphological changes of single cells in the corneal stroma of the living mouse will be observed under a fluorescence microscope. The behavior of normal cells as well as those in the neighborhood of a mild, circumscribed, injury will be followed. The injury will consist of a small circular epithelial debridement or the introduction of hydrogen ions by iontophoresis over a 0.4 mm circle, both of which are reproducible. The keratocytes and other stromal cells will be followed by the use of transgenic mice engineered to express fluorescent proteins ubiquitously, or by staining with nuclear or cytoplasmic fluorescent dyes. Leucocytes will be labeled with nuclear dye systemically. The images are recorded by a digital camera and time-lapse sequences are constructed for analysis. The mechanisms of cell death, activation and repopulation after a mild injury, will be characterized and analyzed. It is intended that further studies will lead to a clarification of the entire wound healing process in the cornea. It has an immediate relevance to the healing of surgical incisions and laser ablations made in the course of refractive surgery. Since the reactions of cells to injury in all tissues is likely to be similar, the results should have a wide significance.

描述（由申请人提供）： 的运动和形态变化 将在活体小鼠角膜基质中的单细胞在 荧光显微镜。正常细胞以及正常细胞的行为 接下来是轻微的、有限的伤害。受伤会 包括一个小的圆形上皮清创术或引入 通过离子电渗疗法在 0.4 毫米圆上检测氢离子，两者都是 可重现。角膜细胞和其他基质细胞之后将是 使用转基因小鼠来普遍表达荧光蛋白， 或通过用核或细胞质荧光染料染色。白细胞将会 用核染料系统标记。图像由数码相机记录 构建相机和延时序列以进行分析。其机制 轻度损伤后的细胞死亡、激活和再生 进行了表征和分析。进一步的研究将导致 阐明角膜的整个伤口愈合过程。它有一个 与手术切口和激光消融的愈合直接相关 在屈光手术过程中进行。由于细胞的反应 所有组织的损伤可能都是相似的，结果应该有广泛的差异 意义。