WOUND HEALING IN THE CORNEAL STROMA

角膜基质的伤口愈合

• 批准号: 2668363
• 负责人: DAVID M MAURICE
• 金额: $ 14.1万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-02-01 至 2000-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2668363
• 关键词: cell biology; cell cycle; cell death; cell migration; cell population study; cell type; confocal scanning microscopy; corneal stroma; electron microscopy; eye injury; fibroblasts; immunocytochemistry; laboratory mouse; light microscopy; phagocytosis; tissue /cell culture; wound healing

1. Tissue culture media will be modified in an attempt to cause tissue cultured corneal endothelial monolayers to acquire their normal functional state as indicated by their electrical properties. 2. Perfusion media will be modified so as to maintain the cornea of a perfused isolated eye in good condition for as long as possible. The behavior of the tissue will be compared with that of an isolated cornea perfused with the same medium, in order to test whether a component secreted by the ciliary body is essential to its welfare. 3. The kinetics of the different cell types within an injured corneal stroma will be followed in order to clarify their origins, transformations and fate. 4. The cellular reactions to corneal injuries in the isolated perfused eye, in the confines of the stroma, and under a glued-on on contact lens will be examined, with a view to identifying the cellular and stimulatory contributions from the blood and tears. 5. Liposomes suspended in physiological medium will be used as indicators of fluid flow across the endothelial surface in order to determine whether the fluid pump operates between or across the cells. 6. An electrochemical potential difference of Na, K, Cl and HCO3 across the endothelium will be looked for by bringing ion- sensitive electrodes close to the cell layer and blocking ion fluxes. The development of a potential step will establish that an ion is involved in an active transport mechanism.

1. 组织培养基将被修改以试图引起 组织培养角膜内皮单层以获得其 由其电气特性指示的正常功能状态。 2. 修改灌注介质以维持角膜 一只处于良好状态的灌注隔离眼只要 可能的。 该组织的行为将与 用相同的介质灌注分离的角膜，以便 测试睫状体分泌的成分是否是必需的 为了它的福祉。 3. 受伤后不同细胞类型的动力学 将跟踪角膜基质以澄清其起源， 转变和命运。 4. 孤立的角膜损伤的细胞反应 灌注的眼睛，在基质的范围内，并在胶粘剂下 将检查隐形眼镜上的情况，以确定 来自血液和眼泪的细胞和刺激贡献。 5. 悬浮在生理介质中的脂质体将用作 流经内皮表面的液体流动指标 确定流体泵是否在之间或跨过 细胞。 6. Na、K、Cl和的电化学势差 穿过内皮的 HCO3 将通过引入离子来寻找 敏感电极靠近细胞层并阻挡离子通量。 潜在步骤的发展将确定离子是 参与主动运输机制。