Core--Immunohistochemistry

核心--免疫组化

• 批准号: 6361823
• 负责人: GREGORY BOSSART
• 金额: $ 17.91万
• 依托单位: UNIVERSITY OF NORTH CAROLINA WILMINGTON
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-07-10 至 2005-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6361823
• 关键词: biomedical facility; chemical kinetics; environmental health; environmental toxicology; immunocytochemistry; marine toxins

Description (Taken from the Investigator's Abstract) The objectives of this core facility are to support three of the proposed projects in this study: Lovelace Institute Rat studies, the Sheep and Rat Animal Model Studies, and the Biomarker and Epidemiology Studies. This facility will provide a variety of services including immunohistochemical staining for toxins, characterization of cellular elements on swabs and scraping and review of histological slides. The immunohistochemistry Core Facility is located within the Division of Comparative Pathology in the Papanicolaou Cancer Research Building. The Division has over 6,000 square feet of laboratory, administrative and office space. There are four full time faculty members and an administrative and support staff of 10 individuals. The laboratories are fully equipped to perform the proposed studies except for the specialized microscope for morphometry, which is included in the budget. The complete histopathology laboratory is available including a Fischer Histomatic model 166MP Tissue Processor, a Shandon Embedding station, two Leitz model 1512 Microtomes, an AO Cryostat, and complete equipment for routine, special stains, and immunohistochemistry. In addition, basic research equipment such as centrifuges (refrigerated/non- refrigerated, and high speed), light microscopes, refrigerators, freezers, autoclaves, C02 incubators, biological safety cabinets, microfuges, ELISA readers, a gamma counter, fume hoods, water baths, fluorescent microscopes, and a walk-in cold room are also available. The shared department resource area also includes a fully equipped laboratory for PCR analysis. Office space is available for participants in this facility that includes their own personal computers with Internet access, multiple programs including Word and Excel, and several data analysis programs.

描述（取自研究者的摘要）该核心设施的目标是支持本研究中提出的三个项目：Lovelace Institute Rat研究，绵羊和大鼠动物模型研究以及生物标志物和流行病学研究。该设施将提供各种服务，包括用于毒素的免疫组织化学染色，拭子上的细胞元素的表征以及刮擦和审查组织学幻灯片。免疫组织化学核心设施位于Papanicolaou癌症研究大楼中比较病理学的划分内。该部门拥有超过6,000平方英尺的实验室，行政和办公空间。有四名全职教职员工以及10个人的行政和支持人员。除了预算中包含的专业形态计量学显微镜外，实验室具有完全有能力进行拟议的研究。可以使用完整的组织病理学实验室，包括Fischer组织模型166MP组织处理器，Shandon嵌入式站，两个Leitz型号1512微失位，AO低温恒温器以及用于常规，特殊污渍和免疫组织化学的完整设备。此外，基础研究设备，例如离心设备（冷藏/非冷藏和高速），轻型显微镜，冰箱，冰柜，高压灭菌器，C02孵化器，生物安全柜，微叶酸还提供浴室，荧光显微镜和步入式寒冷室。共享部门资源领域还包括一个设备齐全的实验室，用于PCR分析。该设施的参与者可以使用办公空间，其中包括其自己的个人计算机，具有互联网访问，包括Word和Excel在内的多个程序以及多个数据分析程序。