CORE--NEUROPATHOLOGY

核心--神经病理学

• 批准号: 6302728
• 负责人: Stephen J. DeArmond
• 金额: $ 20.45万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-12-01 至 2000-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6302728
• 关键词: animal tissue; biomedical facility; information systems; monoclonal antibody; neuropathology; prions; scrapie

The neuropathological phenotype of prion disease is one of the two most commonly used biological parameters used to identify and define prion strains and to characterize the effects of PrP constructs in transgenic mice. The Neuropathology ore provides a neurohistological and immunohistochemical service to the projects, however, it has also made significant contributions to understanding the etiology and pathogenesis of prion diseases. The Aims of the Neuropathology Core are: (1) To sacrifice animals, remove organs, and free or embed tissue blocks in preparation for a variety of morphological studies; (2) To perform classical neurohistological staining and immunohistochemical staining procedures on aldehyde-fixed tissue sections to evaluate the degree of vacuolar degeneration (hematoxylin and eosin stain), to search for amyloid plaques (PAS and CONGO red stains), to evaluate nerve cell loss (Luxol fast blue-PAS stain), and to evaluate the degree of astrocytic gliosis (glial fibrillary acidic protein immunostain); (2) To localize PrP/sc in aldehyde fixed tissue sections pretreated by the hydrolytic autoclaving technique; (3) To localize and quantify PrP/c and PrP/sc in frozen brain sections by the histoblot technique; (4) To fix peripheral nerve in glutaraldehyde, embed in araldite plastic, and prepare toluidine blue stained sections in selected animals to search for possible peripheral neuropathies; (5) To quantify the degree of songiform degeneration, reactive astrocytic gliosis, and PrP/c accumulation by computer-assisted morphometry; (6) To maintain a data base for storage and retrieval of the now several thousand animals which have been processed in part by the Neuropathology Core of this Program Project, as well as, by other Neuropathology Cores which have been part of other prion disease program projects; and (7) To consult with and prepare photomicrographs and graphs to project investigators for publication.

政治疾病的神经病理表型是最重要的两者之一 用于识别和定义prion的常用生物学参数 菌株并表征转基因中PRP构建体的影响 老鼠。神经病理学矿石提供了神经组织学和 但是，对这些项目的免疫组织化学服务也是如此 对理解病因和发病机理的重要贡献 病毒疾病。神经病理学核心的目的是：（1） 牺牲动物，去除器官以及自由或嵌入的组织块 为各种形态学研究做准备； （2）执行 经典的神经组织学染色和免疫组织化学染色 关于醛固定组织切片的程序，以评估 液泡变性（苏木精和曙红染色），以寻找淀粉样蛋白 斑块（PAS和刚果红色污渍），以评估神经细胞的损失（卢克斯 快速蓝-PAS染色），并评估星形细胞神经胶质病的程度 （神经胶质原纤维酸性蛋白免疫抑制剂）； （2）本地化PRP/SC 醛固定组织切片由水解高压剂预处理 技术; （3）在冷冻大脑中定位和量化PRP/C和PRP/SC 组体印迹技术的部分； （4）修复外围神经 戊二醛，嵌入蜡脂塑料中，制备甲醛蓝色 选定动物中的染色切片以寻找可能的外围 神经病； （5）量化歌曲形变性的程度， 反应性星形胶质神经胶质性和PRP/C通过计算机辅助积累 形态计； （6）维护数据库以进行存储和检索 现在，数千只动物部分由 该计划项目的神经病理学核心以及其他 神经病理学核心曾是其他prion疾病计划的一部分 项目； （7）咨询和准备显微照片和图形 向研究人员进行发布。