NEUROPATHOLOGY CORE

神经病理学核心

基本信息

批准号：
7624799
负责人：
Stephen J. DeArmond
金额：
$ 30.84万
依托单位：
UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-04-01 至 2014-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7624799
关键词：
Affect Age of Onset Alleles Alzheimer&apos s Disease Apolipoprotein E Atrophic Autopsy Biocompatible Materials Biological Markers Blood Blood specimen Brain Brain region California Chills Clinical Code Collaborations Creutzfeldt-Jakob Syndrome DNA Data Databases Degenerative Disorder Dementia Dendrites Diagnosis Diagnostic Disease Dissection Enrollment Environment Excision Factor Analysis Fostering Freezing Frontotemporal Lobar Degenerations Genes Genetic Genetic Databases Genetic Materials Genomics Genotype Glial Fibrillary Acidic Protein Goals Graph Heterogeneity Hippocampus (Brain)Histology Human Human Resources Image Immersion Investigative Technique Institution Instruction Laboratories Lead Maintenance Measurement Medical center Methods Microscopic Mission Molecular Molecular Genetics Motor Neuron Disease Nerve Degeneration Neurologist Neurons Neurosciences Other Genetics Pathology Patients Pennsylvania PrPSc Proteins Presynaptic Terminals Principal Investigator Progressive Supranuclear Palsy Proteomics Protocols documentation Publications Publishing Qualifying Radial Recruitment Activity Reporting Research Research Personnel Research Training Resources Risk Sampling San Francisco Scientist Sclerosis Secure Sequence Analysis Services Single Nucleotide Polymorphism Slice Slide Specimen Spinal Cord Staining method Stains Synaptophysin Testing Time Tissues Ubiquitin Universities Update Writing alpha synuclein base brain tissue clinical Diagnosis cohort corticobasal degeneration digital digital imaging dopaminergic neuron genetic analysis genetic risk factor interdisciplinary collaboration interest morphometry multidisciplinary neuropathology novel novel strategies paraform presenilin-1 protein TDP-43 research study tau Proteins tissue processing

项目摘要

Core D, the Neuropathology Core (NP Core), is a continuation of the same core that has served the ADRC for the past 4 years. The personnel are the same except for a recently hired histology technician. Because Dr. Bruce Miller and the Clinical Core have recruited patients with FTDs and atypical dementing disorders throughout Northern California and beyond, we have hired Glen Oaks Pathology Services (San Francisco) to remove brains from enrolled patients within a 150 mile radius from UCSF. The chilled brain is transported to Dr. Eric Huang at the San Francisco VA Medical Center where the first of three brain dissection steps is performed: Dr. Huang coronally sections the fresh brain and alternate slabs are either immersion fixed in paraformaldehyde or frozen. Next, the fixed brain slabs are transported to Dr. William Seeley's laboratory at the UCSF Mission Center Building (MCB). Dr. Seeley has been studying selective neuronal vulnerability in FTD in close collaboration with Dr. DeArmond and the NP Core. Dr. Seeley dissects brain regions of interest for his studies and also performs dissections for other ADRC investigators. Finally, the remaining fixed brain samples are sent to Dr. DeArmond's Lab (adjacent to Dr. Seeley's) for removal of ~30 blocks of tissue for diagnostic neuropathological analysis. Dr. DeArmond and his staff perform multiple neurohistological and immunohistochemical stains on the blocks, perform semiquantitiatve analysis of the stained slides, write a detailed neuropathology final report on each case, and complete a NACC form. The NP Core provides quality digital photographs and quantitative morphometric analysis of neuropathological changes as needed for publications. The overall aim of this ADRC is to better understand the clinical heterogeneity of patients with AD, FTD and related disorders, and to tackle the problem of selective vulnerability. Complimentary to the clinical aim, the overall aim of the NP Core is to better understand the overlap of multiple different clinically and neuropathologically defined entities we are finding in many dementia patients. Through a consortium arrangement with Dr. Daniel Geschwind at UCLA, the NP Core will oversee transport of blood samples to his lab for differential genetic analysis of heterogeneity. RELEVANCE (See instructions): This core will provide a unique resource to dementia neuroscience investigators at the UCSF ADRC and beyond by systematically banking brain tissue and genetic materials from patients with AD, FTD, and other dementias. These resources are intended to facilitate modern neuroscientific research studies related to these disorders that will ultimately lead to new treatments.

核心D，神经病理学核心（NP核心），是为ADRC服务的相同核心的延续在过去的四年中。除最近雇用的组织学技术人员外，人员是相同的。因为布鲁斯·米勒（Bruce Miller）博士和临床核心已招募了FTD和非典型痴呆症患者在整个北加州及其他地区，我们聘请了Glen Oaks病理学服务（旧金山）从UCSF半径150英里内，从注册患者中清除大脑。冷藏大脑被运输到旧金山弗吉尼亚州医疗中心的埃里克·黄博士在三个大脑解剖中的第一个是进行的：黄冠冕博士的新鲜大脑和替代板固定在多聚甲醛或冷冻。接下来，将固定的脑板运送到William Seeley博士的实验室 UCSF任务中心大楼（MCB）。 Seeley博士一直在研究选择性神经元脆弱性 FTD与Dearmond博士和NP Core密切合作。 Seeley博士剖析了感兴趣的大脑区域为了他的学习，还为其他ADRC研究人员进行解剖。最后，剩余的固定大脑样品被发送到迪尔蒙德博士的实验室（靠近Seeley博士），以取消约30个块的组织诊断神经病理分析。迪尔蒙德博士和他的员工进行多种神经组织学和块上的免疫组织化学染色，对彩色幻灯片进行半定位分析，写一个详细的关于每种情况的神经病理学最终报告，并填写NACC表格。 NP核心提供根据需要出版物。该ADRC的总体目的是更好地了解患者的临床异质性使用AD，FTD和相关疾病，并解决选择性脆弱性的问题。补充临床目的，NP核心的总体目的是更好地了解多个不同的重叠我们在许多痴呆症患者中发现的临床和神经病理学定义的实体。通过一个与UCLA的Daniel Geschwind博士的财团安排，NP核心将监督血液的运输样品到他的实验室进行异质性差异遗传分析。相关性（请参阅说明）：该核心将为UCSF ADRC和除了系统地将AD，FTD患者和其他患者的脑组织和遗传材料融为一体之外痴呆症。这些资源旨在促进与现代神经科学研究研究这些疾病最终会导致新治疗。