PERMSELECTIVITY OF MYOCARDIAL CAPILLARIES (EFFECTS OF ELECTROKINETICS)

心肌毛细血管的通透性（电动效应）

• 批准号: 6308532
• 负责人: JAMES B BASSINGTHWAIGHTE
• 金额: $ 2.35万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-12-01 至 2000-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6308532
• 关键词: Mammalia; bioengineering /biomedical engineering; biomedical resource; human tissue; technology /technique

An idealized continuum model of electrokinetic transport is developed to analyze the contributions of solute and barrier charge to microvascular permeability. The glycocalyx fiber-matrix-filled interendothelial cleft of the transcapillary exchange barrier is modelled with parallel plates representing endothelial cell surfaces and a bridging regular array of identical cylinders representing glycocalyx fibers. To calculate the electrostatic potential within the confined fiber-matrix, the linear Poisson-Boltzmann equation is solved for a single bounded post and a regular array of bounded posts using several analytical methods (separation of variables, Green's functions and linear superposition and unit cell approximations) and numerical methods (direct formulation of a boundary element method and boundary collocation method). The potential decays monotonically with distance from the surfaces vanishing between four and five Debye lengths. For values typical of plasma ultrafiltrate in a glycocalyx filled interendothelial cleft, namely a Debye length of 8 A , plate separation of 120 A, post radius of 6 A and surface potential of 60 mV, the potential remains nonzero for porosity values less than 97%. Thus, throughout the confined matrix region of this idealized model, there is enhanced anionic and diminished cationic convective transport compared to neutral molecules. The effect is more pronounced for larger molecules whose double layers extend into double layer regions close to the post or plates where the potential magnitude is greatest. This result agrees with published experimental observations of transport of charged molecules.

动电传输的理想化连续模型是 开发用于分析溶质和势垒电荷的贡献 微血管通透性。 糖萼纤维基质填充 跨毛细血管交换屏障的内皮间裂是 用代表内皮细胞表面的平行板建模 和相同圆柱体的桥接规则数组代表 糖萼纤维。 计算静电势 受限纤维矩阵，线性泊松-玻尔兹曼方程为 解决了单个有界帖子和有界帖子的常规数组 使用多种分析方法（变量分离、格林法 函数和线性叠加和晶胞近似）和 数值方法（边界元法的直接公式化和 边界配置法）。 电势单调衰减 距表面的距离在四到五德拜之间消失 长度。 对于糖萼中血浆超滤液的典型值 填充的内皮间隙，即德拜长度为 8 A ，板 分离 120 A，柱半径 6 A，表面电位 60 mV，当孔隙率值小于 97% 时，电势保持非零。 因此，在这个理想化模型的整个有限矩阵区域中， 阴离子对流传输增强，阳离子对流传输减弱 与中性分子相比。 对于以下情况效果更为明显 双层延伸至双层区域的较大分子 靠近潜在震级最大的柱子或板。 该结果与已发表的实验观察一致 带电分子的运输。