CORE--ORGANIC SYNTHESIS FACILITY

核心——有机合成装置

• 批准号: 6300127
• 负责人: SHANTU G AMIN
• 金额: $ 26.2万
• 依托单位: INSTITUTE FOR CANCER PREVENTION
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-12-22 至 2000-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6300127
• 关键词: biomedical facility; carcinogens; chemical synthesis; organic chemicals

The Organic Synthesis Facility supports the Cancer Center's research activities in environmental carcinogenesis, and chemoprevention, and the identification of host factors of etiologic significance by providing chemically pure compounds for model assays, as reference materials, and as internal standards. It is a dedicated resource occupying 900 sq. ft. in Unit 1 and 447 sq. ft on the lower level of the NDI in Valhalla. The CCSG- supported staff are the Facility Head, Shantu Amin, Ph.D. and one organic chemist, Jacek Krzeminski, Ph.D. The Facility supports the Cancer Center's research activities in environmental carcinogenesis, and chemoprevention, and the identification of host factors of etiologic significance by providing chemically pure compounds for model assays, as reference materials, and as internal standards. Requested compounds are furnished to AHFCC's investigators in compounds is ensured by state-of-the-art technology, including HPLC, GC-MS, TLC, UV, IR, FTIR, MS, and NMR. The scientists in the facility are highly experienced in developing efficient synthetic routes for cold and radioisotope-labeled polynuclear aromatic hydrocarbons, tobacco-specific nitrosamines, and other environmental and nutritional carcinogens as well as their metabolites, and their DNA and protein adducts. The Facility's dedicated Carcinogen Synthesis Laboratory is equipped with high pressure liquid chromatographs including one instrument with a beta- flo-one model IC radioactivity detector, and with a medium pressure liquid chromatograph. These instruments enable the analysis and purification of starting materials, intermediates, and end products. The laboratory has hoods with glove box, a walk-in hood as required by regulatory statutes, and a special photolysis set-up. The dedicated selenium laboratory, in which chemoprevention agents are prepared, has a special exhaust system in compliance with safety regulations pertaining to these chemicals. The dedicated Synthesis Laboratory for intermediates, nucleoside markers, and chemopreventive agents is equipped with a photolysis set-up. HPLC with photo diode array detector, and two working hoods with a 24-hour exhaust system. Radiolabeled syntheses are carried out in another dedicated laboratory that is accessed only upon approval and is also supervised by the AHFCC's Safety Officer.

有机合成设施支持癌症中心的研究 环境致癌和化学预防方面的活动，以及 通过提供鉴定具有病因学意义的宿主因素 用于模型分析的化学纯化合物，作为参考材料，以及 内部标准。这是一个占地 900 平方英尺的专用资源。 瓦尔哈拉 (Valhalla) NDI 下层 1 号单元，面积 447 平方英尺。 CCSG- 支持的工作人员是设施负责人 Shantu Amin 博士。和一种有机物 化学家 Jacek Krzeminski 博士该设施支持癌症中心的 环境致癌和化学预防方面的研究活动， 以及通过以下方法鉴定具有病因学意义的宿主因素： 为模型分析提供化学纯化合物，作为参考 材料，并作为内部标准。请求的化合物提供给 AHFCC 的化合物研究人员得到最先进的保证 技术，包括 HPLC、GC-MS、TLC、UV、IR、FTIR、MS 和 NMR。这 该设施的科学家在开发高效 冷和放射性同位素标记的多核芳香族化合物的合成路线 碳氢化合物、烟草特有的亚硝胺以及其他环境和 营养致癌物及其代谢物及其 DNA 和 蛋白质加合物。 该设施的专用致癌物合成实验室配备了 高压液相色谱仪，包括一台带有 beta- 的仪器 flo-one型IC放射性检测仪，并配有中压液体 色谱仪。这些仪器能够分析和纯化 原材料、中间体和最终产品。实验室有 带手套箱的抽油烟机、监管法规要求的步入式抽油烟机、 和特殊的光解装置。专门的硒实验室，位于 配制化学预防剂，有特殊的排气系统 遵守与这些化学品有关的安全法规。这 中间体、核苷标记物的专用合成实验室 化学预防剂配备有光解装置。高效液相色谱法 光电二极管阵列探测器和两个带 24 小时排气功能的工作罩 系统。放射性标记的合成在另一个专用的 实验室只有在获得批准后才能进入，并受到以下机构的监督 AHFCC 的安全官员。