CORE--DNA/PEPTIDE

核心--DNA/肽

• 批准号: 6334938
• 负责人: ROBERT W SCHACKMANN
• 金额: $ 8.47万
• 依托单位: UNIVERSITY OF UTAH
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-07-25 至 2001-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6334938
• 关键词: biomedical facility; nucleic acid chemical synthesis; nucleic acid sequence; peptide chemical synthesis; protein sequence

Oligonucleotides, peptides, and protein sequencing are essential tools of modern molecular medicine and biology. Access to these tools is essential for investigation and diagnosis of genetic alterations linked to transformed cell states and to many infectious diseases. The DNA/Peptide Shared Resource provides a dependable, rapid, and affordable supply of synthetic DNA and RNA (oligonucleotides), peptides, and protein sequencing for Cancer Center members. Oligonucleotides are used in DNA sequencing, as polymerase chain reaction primers, for site-directed mutagenesis, and as probes for the identification of genetic information Modified oligonucleotides have been used for anti-sense experiments with direct therapeutic possibilities. Synthetic peptides are used as a direct means of exploring the function of, or to generate immunological agents against, specific regions of proteins. Protein sequencing is used to confirm the identify of proteins or to identify regions of amino acid sequence in proteins of interest. This facility provides these services in a fast, efficient manner that minimizes the cost to individual laboratories. It also provides low cost access to our instrumentation for researchers at the Center, should they develop reagents or techniques requiring it. This facility serves as an information source for Cancer Center members to find assistance with the design and implementation of specific types of experiments. Staff members are available to train Cancer Center members in a wide variety of DNA/Peptide techniques.

寡核苷酸，肽和蛋白质测序是现代分子医学和生物学的重要工具。使用这些工具对于研究和诊断与转化的细胞状态以及许多传染病有关的遗传改变至关重要。 DNA/肽共享资源为癌症中心成员提供了合成DNA和RNA（寡核苷酸），肽和蛋白质测序的可靠，快速和负担得起的供应。寡核苷酸用于DNA测序，作为聚合酶链反应引物，用于定位诱变，作为鉴定遗传信息修饰的寡核苷酸的探针，已用于具有直接治疗可能性的抗敏感实验。合成肽被用作探索蛋白质特定区域的免疫学剂的功能或产生免疫学剂的功能的直接手段。蛋白质测序用于确认蛋白质的识别或鉴定感兴趣蛋白质中氨基酸序列的区域。该设施以快速，高效的方式提供这些服务，以最大程度地减少各个实验室的成本。它还为中心的研究人员提供了低成本的访问，如果他们开发了需要它的试剂或技术。该设施是癌症中心成员的信息来源，以在特定类型的实验的设计和实施方面找到帮助。工作人员可以用各种DNA/肽技术培训癌症中心成员。