Core--DNA and Peptide Synthesis

核心--DNA与多肽合成

• 批准号: 6990200
• 负责人: ROBERT W SCHACKMANN
• 金额: $ 1.45万
• 依托单位: UNIVERSITY OF UTAH
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-07-09 至 2006-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6990200
• 关键词: biomedical facility; chemical registry /resource; neoplasm /cancer; nucleic acid chemical synthesis; peptide chemical synthesis; synthetic nucleic acid; synthetic peptide

Oligonucleotides, peptides and protein sequencing are essential tools of modem molecular medicine and biology. Access to these tools is essential for investigation and diagnosis of genetic alterations linked to transformed cell states and many infectious diseases. The DNA/Peptide Shared Resource provides a dependable, rapid and affordable supply of synthetic DNA and RNA (oligonucleotides), peptides and protein sequencing for cancer center members and others in the research community at the University of Utah. Oligonucleotides are used in DNA sequencing, as polymerase chain reaction primers, for site-directed mutagenesis, and as probes for the identification of genetic information. Modified oligonucleotides have been used for antisense experiments and gene silencing experiments with therapeutic possibilities. Synthetic peptides are used as a direct means of exploring the function of, or to generate ilmnunological reagents against, specific regions of proteins. Protein sequencing is used to confirm the identity or start sites of proteins or to identify regions of amino acid sequence in proteins of interest. This facility provides these services in a fast, efficient manner that minimizes the cost and risk to individual laboratories. It also provides access to instrumentation should cancer center members develop reagents or techniques requiring them. Finally, the facility serves as an information source for cancer center members using a wide variety of DNA/Peptide techniques.

寡核苷酸，肽和蛋白质测序是现代分子医学和生物学的重要工具。使用这些工具对于研究和诊断与转化的细胞状态和许多传染病有关的遗传改变至关重要。 DNA/肽共享的资源为癌症中心成员以及犹他大学研究界的其他合成DNA和RNA（寡核苷酸），肽和蛋白质测序提供了可靠，快速和负担得起的供应。 寡核苷酸用于DNA测序，作为聚合酶链反应引物，定向诱变，作为鉴定遗传信息的探针。修饰的寡核苷酸已用于具有治疗可能性的反义实验和基因沉默实验。合成肽被用作探索蛋白质特定区域的ILMNONOLOGY试剂的功能或产生ILMNONOLOGY试剂的直接手段。蛋白质测序用于确认蛋白质的同一性或起始位点或鉴定感兴趣蛋白质中氨基酸序列的区域。该设施以快速，高效的方式提供这些服务，以最大程度地减少对个别实验室的成本和风险。如果癌症中心成员开发需要它们的试剂或技术，它还可以提供仪器的访问。 最后，该设施是使用多种DNA/肽技术的癌症中心成员的信息来源。