Fluorescent Electronic Nose Finds Environmental Exposure

荧光电子鼻发现环境暴露

• 批准号: 6400258
• 负责人: EUGENE C AQUINO
• 金额: $ 20万
• 依托单位: AMERICAN RESEARCH CORP OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-30 至 2002-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6400258
• 关键词: air pollution; air sampling /monitoring; biomarker; biomedical equipment development; breath tests; environmental exposure; fluorescent dye /probe; fluorimetry; monitoring device; portable biomedical equipment

DESCRIPTION (provided by applicant): Volatile organic compounds (VOCs) have been implicated in degradation of human physiological systems and in the etiology of disease. At present, monitoring breath-based biomarkers of exposure to VOCs requires specialized instrumentation such as gas chromatography with mass spectroscopy or photoionization detection. Recent developments in the application of "electronic nose" systems have been limited by part-per-million levels of detection. There is a need to develop low-cost, portable, array sensors capable of rapid detection of breath components at low part-per-billion (ppb) concentrations. To meet this need, American Research Corporation of Virginia suggests the development of a confocal array fluorimeter-based chemical sensor for use with chemometric calibration and analysis. The proposal is innovative based on the combination of a sensitive miniaturized photodiode array with selective fluorophore-polymer panels to enable rapid, sensitive detection of VOC biomarkers. Specific Aims of the program include evaluation of fluorescent membrane compositions suitable for detection of breath biomarkers, modification of a confocal fluorimeter for multiple channel detection, acquisition of families of test data from simulated breath exhalant and optimization of an engineering model for the Phase II integrated sensor. The program is significant in providing a customizable, rugged and lower cost analytical instrument that is sensitive to breath biomarkers associated with exposure to volatile organic compounds. PROPOSED COMMERCIAL APPLICATION: Successful completion of the Phase I program will result in a miniaturized fluorescent array detector to monitor the presence of volatile organic compounds in human breath. The chemical detector will find commercial use as an "electronic nose" for use in environmental chemical analysis and in food processing industries.

描述（由申请人提供）： 挥发性有机化合物（VOC）与人类的退化有关 生理系统和疾病的病因学。 目前，监测 基于呼吸的 VOC 暴露生物标志物需要专门的 仪器，例如带有质谱的气相色谱或 光电离检测。 应用的最新进展 “电子鼻”系统受到百万分之一的水平的限制 检测。 需要开发低成本、便携式阵列传感器 能够快速检测十亿分之一 (ppb) 的呼吸成分 浓度。 为了满足这一需求，弗吉尼亚美国研究公司 建议开发基于共焦阵列荧光计的化学传感器 用于化学计量校准和分析。 该提案具有创新性 基于灵敏的小型化光电二极管阵列与 选择性荧光团聚合物面板可实现快速、灵敏的检测 VOC 生物标志物。 该计划的具体目标包括评估 适用于检测呼吸生物标志物的荧光膜组合物， 改进共焦荧光计以进行多通道检测， 从模拟呼吸呼出物中获取测试数据族 二期集成传感器工程模型的优化。 这 该计划对于提供可定制、坚固且成本更低的产品具有重要意义 对呼吸生物标志物敏感的分析仪器 接触挥发性有机化合物。 拟议的商业应用： 第一阶段计划的成功完成将产生小型荧光阵列探测器，用于监测人类呼吸中挥发性有机化合物的存在。该化学探测器将作为“电子鼻”投入商业用途，用于环境化学分析和食品加工行业。