SEQUENCING COMNPONENT

测序组件

• 批准号: 6109127
• 负责人: William Richard McCombie
• 金额: $ 20万
• 依托单位: COLD SPRING HARBOR LABORATORY
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-18 至 2000-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6109127
• 关键词: bacteriophage M13; biomedical automation; biotechnology; chromosomes; genetic library; genetic mapping; genome; human genetic material tag; molecular cloning; nucleic acid sequence; optics; polymerase chain reaction

We propose to establish a high throughput sequencing team capable of efficiently producing 50-100 megabases of high quality genomic sequence per year at a competitive cost. The essential elements of any high throughput sequencing center are an infrastructure of experienced sequencing personnel, software, and equipment that can efficiently produce DNA sequence data. We will establish such a center with an emphasis on quality at all levels of the sequencing process from clone verification to the correct determination of each specific base. We propose in a three year period to expand to a level that would allow us in the subsequent 1-2 years to reach 50-100 megabases of sequence if this were needed programmatically. We further propose to sequence 3 megabases in year 1,9 megabases in year 2, and 24 megabases in year 3. This will put us in a position to fish the sequence of chromosome 18 prior to the target date for schedule completion of the first reference sequence. We also propose to use Optical Mapping to facilitate the assembly of sequence, especially in difficult regions of the genome. Having the detailed structure of a region often provides clues to the order of contigs during assembly. This will facilitate linking of contigs as well as suggest strategies for the closure of gaps based on the estimated gap size. We will also use these maps to verify the completed assemblies as part of our quality control program.

我们建议建立一个能够 有效产生高质量基因组序列的50-100兆瓦 每年的竞争成本。任何高的基本要素 吞吐量测序中心是经验丰富的基础设施 可以有效生产的测序人员，软件和设备 DNA序列数据。我们将建立这样一个中心，重点 从克隆验证到测序过程的各个级别的质量 每个特定基础的正确确定。我们提出三个 一年期扩展到将使我们在随后的1-2中的水平 如果需要的话，可以达到50-100兆袋的序列 以编程方式。我们进一步提议在1，9年对3个大兆巴进行序列序列 2年级的巨型公布和第3年的24个巨蛋。这将使我们陷入 在目标日期之前钓鱼18染色体的序列的位置 为了完成第一个参考顺序的时间表。我们也建议 使用光学映射来促进序列的组装，尤其是 在基因组的困难地区。具有详细的结构 区域通常为组装过程中重叠群的顺序提供线索。这 将促进重叠群的联系，并提出策略 基于估计的间隙大小的差距闭合。我们还将使用这些 作为我们质量控制的一部分，地图验证完成的组件 程序。