CORE--MOLECULAR BIOLOGY

核心--分子生物学

• 批准号: 6099443
• 负责人: Richard D Kolodner
• 金额: $ 11.77万
• 依托单位: DANA-FARBER CANCER INST
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-08-01 至 1999-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6099443
• 关键词: AIDS; DNA binding protein; aminoacid analyzer; biomedical facility; biosensor device; computer assisted sequence analysis; electron microscopy; high performance liquid chromatography; nucleic acid chemical synthesis; nucleic acid sequence; oligonucleotides; peptide chemical synthesis; protein sequence; synthetic peptide

The Dana-Farber Cancer Institute has established a Molecular Biology Core Facility to help Institute laboratories more easily and cost effectively use molecular biology techniques and reagents such as oligonucleotides, synthetic peptides, microprotein sequencing, mass analysis, DNA sequencing, protein-ligand assays and electron microscopy of nucleic acids and nucleic acid-protein interactions. This application requests funds to partially cover costs not covered by a charge back system. The facility contains the following major equipment: Applied Biosystems 394 DNA/RNA Synthesizer, Applied Biosystems 380B DNA Synthesizer, Applied Biosystems 477 Protein Sequencer, 120A Analyzer, Applied Biosystems 430 Peptide Synthesizer, Hewlett Packard HP1090 Micropore Liquid Chromatograph, 2 Waters HPLC's Applied Biosystems 420/H-130 Amino Acid Analyzer, Finnigan Lasermat TOF Mass Analyzer, Pharmacia BIAcore biosensor, Applied Biosystems 373 DNA Sequencer, Zeiss EM 10A Electron Microscope. The facility is staffed by a facility director and 4 full time technicians who provide the different services as well as provided instruction to investigators who wish to use specific instruments themselves.

达纳 - 弗伯癌研究所已经建立了分子生物学核心 设施可帮助更轻松地建立实验室，并有效地成本 使用分子生物学技术和试剂，例如寡核苷酸， 合成肽，微蛋白测序，质量分析，DNA 核的测序，蛋白质 - 配体测定和电子显微镜 酸和核酸 - 蛋白质相互作用。 此申请请求 资金以部分支付费用后部系统覆盖的部分费用。 这 设施包含以下主要设备：应用生物系统394 DNA/RNA合成器，应用生物系统380B DNA合成器，应用 生物系统477蛋白序列仪，120A分析仪，应用生物系统430 肽合成器，惠普Packard HP1090微孔液体 色谱仪，2 Waters HPLC的应用生物系统420/H-130氨基酸 分析仪，Finnigan Lasermat TOF质量分析仪，Pharmacia biacore 生物传感器，应用生物系统373 DNA Sequencer，Zeiss EM 10A电子 显微镜。 该设施由设施主任和4个完整的配备 提供不同服务以及提供的时间技术人员 向希望使用特定工具的调查人员的指导 自己。