CORE--BRAIN BANK RESOURCE

核心——脑库资源

• 批准号: 6098652
• 负责人: David M. Armstrong
• 金额: --
• 依托单位: MCP HAHNEMANN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-11-11 至 1998-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6098652
• 关键词: Alzheimer's disease; aging; basal ganglia; biomedical facility; brain; brain imaging /visualization /scanning; brain stem; cerebellum; cerebral cortex; cryoprotective agents; hippocampus; human tissue; immunocytochemistry; nervous system disorder diagnosis; neural degeneration; postmortem; spinal cord; tissue /cell preparation; tissue resource /registry

The Brain Bank Core Resource will provide complete brain banking and neuropathology diagnostic services for all participants within the AHERF Institute on Aging. Autopsied brain material will be collected under the direction of a board-certified neuropathologist from hospitals located on the Pittsburgh and Philadelphia campuses of the Medical College of Pennsylvania/Hahnemann University. The brain will be bisected sagitally at the time of autopsy. The right half will be dissected for flash-frozen banking and distribution to participating investigators. The left half of the brain will be fixed in buffered formalin. Sections from the cerebral cortex and white matter, hippocampus, basal ganglia, cerebellum, brain stem, and spinal cord will be embedded in paraffin for microscopic examination. Paraffin sections will be routinely stained with hematoxylin and eosin. Special stains may include the Luxol fast blue periodic acid Schiff stain, the Bielschowsky silver stain, a trichrome stain, and a number of immunocytochemical stains (e.g., GFAP, beta A4, PHF). In addition to the differential diagnostics for neurodegenerative diseases, those brains found to possess changes of Alzheimer's disease will be staged according to published criteria of tangle densities. The brain bank will be catalogued and correlated with neuropathology diagnoses to make available well characterized tissues to participating AHERF investigators. Neuropathology diagnoses will be supplied to an AHERF Institute on Aging database. Based upon the analysis of autopsy reports from the past 2 years we can anticipate approximately 80 brains per year from adults with medical and surgical illnesses and no history of dementia or other complicating neurologic disease. Of those 80 brains per year approximately one-half will most likely be autopsied within 12 hours of death. Importantly, our retrospective analyses indicate that we should expect brains from adults ranging in age from more than 20-100 years of age. The availability of brain material from patients representing each decade of life provides a valuable resource to address aged-related questions. With the initiation of the Brain Bank Core Resource these control brains, as well as those with the diagnosis of Alzheimer's disease, will be banked and characterized by routine gross and microscopic neuropathologic examination. In addition, the core will maintain a reference laboratory for development of detailed and quantitative analyses.

脑库核心资源将提供完整的脑库和 为 AHERF 内的所有参与者提供神经病理学诊断服务 老龄化研究所。尸检脑材料将根据 来自位于以下医院的经过委员会认证的神经病理学家的指导 在匹兹堡和费城医学院的校园 宾夕法尼亚/哈尼曼大学。大脑将被矢状一分为二 在尸检时。右半部分将被解剖进行速冻 银行和分发给参与调查员。左半边 大脑的部分将被固定在缓冲福尔马林中。部分来自 大脑皮层和白质、海马、基底神经节、小脑、 脑干和脊髓将包埋在石蜡中进行显微镜检查 考试。石蜡切片将常规用苏木精染色 和曙红。特殊染色剂可能包括 Luxol 固蓝高碘酸 Schiff 染色剂、Bielschowsky 银染色剂、三色染色剂和 免疫细胞化学染色的数量（例如 GFAP、β A4、PHF）。在 除了神经退行性疾病的鉴别诊断之外， 那些被发现患有阿尔茨海默病的大脑将 根据公布的缠结密度标准进行。大脑 银行将被编目并与神经病理学诊断相关联 为参与的 AHERF 提供特征明确的组织 调查人员。神经病理学诊断将提供给 AHERF 老龄化研究所数据库。根据尸检报告的分析 从过去的两年来看，我们预计每年大约有 80 个大脑 来自患有内科和外科疾病且无病史的成年人 痴呆或其他复杂的神经系统疾病。每 80 个大脑中 年约一半的人很可能会在 12 小时内进行尸检 死亡。重要的是，我们的回顾性分析表明我们应该 预计年龄在 20 至 100 岁之间的成年人的大脑 年龄。代表每个患者的大脑材料的可用性 生命的十年为解决与老年相关的问题提供了宝贵的资源 问题。随着脑库核心资源的启动，这些 控制大脑，以及那些被诊断患有阿尔茨海默病的人 疾病，将被存储并通过常规总和和特征 显微镜神经病理学检查。此外，该核心还将 维持一个参考实验室，以制定详细和 定量分析。