CORE--BRAIN BANK RESOURCE

核心——脑库资源

• 批准号: 6201036
• 负责人: David M. Armstrong
• 金额: $ 13.26万
• 依托单位: LANKENAU INSTITUTE FOR MEDICAL RESEARCH
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-01 至 2002-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6201036
• 关键词: Alzheimer's disease; aging; basal ganglia; biomedical facility; brain; brain imaging /visualization /scanning; brain stem; cerebellum; cerebral cortex; cryoprotective agents; hippocampus; human tissue; immunocytochemistry; nervous system disorder diagnosis; neural degeneration; postmortem; spinal cord; tissue /cell preparation; tissue resource /registry

The Brain Bank Core Resource will provide complete brain banking and neuropathology diagnostic services for all participants within the AHERF Institute on Aging. Autopsied brain material will be collected under the direction of a board-certified neuropathologist from hospitals located on the Pittsburgh and Philadelphia campuses of the Medical College of Pennsylvania/Hahnemann University. The brain will be bisected sagitally at the time of autopsy. The right half will be dissected for flash-frozen banking and distribution to participating investigators. The left half of the brain will be fixed in buffered formalin. Sections from the cerebral cortex and white matter, hippocampus, basal ganglia, cerebellum, brain stem, and spinal cord will be embedded in paraffin for microscopic examination. Paraffin sections will be routinely stained with hematoxylin and eosin. Special stains may include the Luxol fast blue periodic acid Schiff stain, the Bielschowsky silver stain, a trichrome stain, and a number of immunocytochemical stains (e.g., GFAP, beta A4, PHF). In addition to the differential diagnostics for neurodegenerative diseases, those brains found to possess changes of Alzheimer's disease will be staged according to published criteria of tangle densities. The brain bank will be catalogued and correlated with neuropathology diagnoses to make available well characterized tissues to participating AHERF investigators. Neuropathology diagnoses will be supplied to an AHERF Institute on Aging database. Based upon the analysis of autopsy reports from the past 2 years we can anticipate approximately 80 brains per year from adults with medical and surgical illnesses and no history of dementia or other complicating neurologic disease. Of those 80 brains per year approximately one-half will most likely be autopsied within 12 hours of death. Importantly, our retrospective analyses indicate that we should expect brains from adults ranging in age from more than 20-100 years of age. The availability of brain material from patients representing each decade of life provides a valuable resource to address aged-related questions. With the initiation of the Brain Bank Core Resource these control brains, as well as those with the diagnosis of Alzheimer's disease, will be banked and characterized by routine gross and microscopic neuropathologic examination. In addition, the core will maintain a reference laboratory for development of detailed and quantitative analyses.

大脑银行核心资源将提供完整的大脑银行业务和 AHERF中所有参与者的神经病理学诊断服务 老化研究所。尸检将在 来自位于医院的董事会认证神经病理学家的方向 在匹兹堡和费城医学院的校园 宾夕法尼亚州/哈尼曼大学。大脑将被暂时划分 在尸检时。右半部分将用于闪光灯 银行和分配给参与的调查员。左半 大脑的福尔马林将固定。来自 大脑皮层和白质，海马，基底神经节，小脑， 脑干和脊髓将嵌入石蜡中以进行微观 考试。石蜡切片将常规用苏木精染色 和曙红。特殊污渍可能包括Luxol快速蓝色周期酸 Schiff Stain，Bielschowsky银色污渍，三色染色和 免疫细胞化学污渍的数量（例如GFAP，Beta A4，PHF）。在 除了神经退行性疾病的差异诊断， 那些发现具有阿尔茨海默氏病变化的大脑将是 根据已发表的纠缠密度标准进行。大脑 银行将被分类并与神经病理学诊断相关联 可以提供充分表征的组织参加AHERF 调查人员。神经病理学诊断将提供给AHERF 老化数据库研究所。基于尸检报告的分析 从过去的两年开始，我们可以预计每年大约有80个大脑 来自患有医疗和外科疾病的成年人，没有 痴呆或其他复杂的神经系统疾病。在这80个大脑中 大约一半的一年很可能会在12小时内进行尸检 死亡。重要的是，我们的回顾性分析表明我们应该 期望成年人的大脑年龄超过20-100岁 年龄。代表每个患者的脑材料的可用性 十年的生命提供了一种宝贵的资源来解决与老年有关的问题 问题。随着Brain Bank核心资源的启动 控制大脑以及有诊断的阿尔茨海默氏症的大脑 疾病，将以常规的总和为特征和特征 微观神经病理学检查。此外，核心将 维护一个参考实验室，以开发详细的和 定量分析。