CORE--MR SPECTROSCOPY

核心--MR光谱

• 批准号: 6241228
• 负责人: MICHAEL B SMITH
• 金额: $ 11.69万
• 依托单位: PENNSYLVANIA STATE UNIV HERSHEY MED CTR
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-07-01 至 1998-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6241228
• 关键词: acidity /alkalinity; bioenergetics; biomedical facility; brain injury; brain mapping; brain metabolism; cerebral ischemia /hypoxia; divalent cations; image processing; magnesium; magnetic resonance imaging; method development; nuclear magnetic resonance spectroscopy; perinatal

The overall objective of the MR Spectrometry Core (C) is to develop and apply multinuclear Nuclear Magnetic Resonance (NMR) spectrometry and imaging core facilities for the study of hypoxic-ischemic brain damage in immature experimental animals. The spectroscopy component of the Core will develop NMR spectroscopy techniques to evaluate metabolic alterations which occur during and following cerebral hypoxia-ischemia in immature rats and newborn dogs. Spatially localized 31P and 1H NMR spectroscopy will be used to quantitate changes in lactate concentrations, energy metabolism, intracellular pH and free cytosolic magnesium concentrations. We have developed a new iterative method to determine more accurately intracellular pH and free magnesium (Mg++) concentrations using the 3 resonances of ATP. The imaging component of the Core will develop and apply magnetic resonance imaging (MRI) techniques to evaluate perinatal hypoxic-ischemic brain damage. Central to the research will be the characterization of the normal developing rat brain. Two imaging techniques will be used to elucidate the sites and severities of brain damage at different states of hypoxia-ischemia and recovery. These techniques include diffusion and T2-weighted spin echo imaging. Preliminary results indicate that diffusion imaging is a sensitive method for early detection of hypoxic- ischemic injury, while T2-weighted spin echo imaging more accurately monitors long-term damage. Animal movement restraining devices essential to obtaining reliable diffusion images will be continually designed, constructed and refined. These devices will be incorporated into the general probe design for spectroscopy or imaging for each research project (Projects 1,3,4)

MR光谱核心（C）的总体目标是发展和 应用多核核磁共振（NMR）光谱法和 成像研究缺氧 - 缺血性脑损伤的核心设施 在不成熟的实验动物中。 核心的光谱分量 将开发NMR光谱技术来评估代谢 在脑缺氧 - 缺血性疾病期间和之后发生的改变 在未成熟的大鼠和新生狗中。 空间本地化的31p和1H NMR 光谱法将用于定量乳酸的变化 浓度，能量代谢，细胞内pH和游离胞质 镁浓度。 我们已经开发了一种新的迭代方法 确定更准确的细胞内pH和游离镁（mg ++） 使用ATP的3个共振的浓度。 核心的成像成分将发展并施加磁性 共振成像（MRI）技术以评估围产期低氧缺血性 脑损伤。 研究的中心将是 正常发育的大鼠大脑。 两种成像技术将用于 阐明不同状态下脑损伤的部位和严重性 缺氧 - 异常恢复和恢复。 这些技术包括扩散和 T2加权自旋回声成像。 初步结果表明 扩散成像是一种敏感的方法，用于早期检测低氧 - 缺血性损伤，而T2加权自旋回波更准确地成像 监视长期损坏。 动物运动限制设备对于获得可靠 扩散图像将不断设计，构造和完善。 这些设备将合并到一般探针设计中 每个研究项目的光谱或成像（项目1,3,4）