CORE--MR SPECTROSCOPY /IMAGING FACILITY

核心——MR光谱/成像设备

• 批准号: 6318360
• 负责人: MICHAEL B SMITH
• 金额: $ 15.82万
• 依托单位: PENNSYLVANIA STATE UNIV HERSHEY MED CTR
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-07-01 至 2001-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6318360
• 关键词: acid base balance; bioenergetics; bioimaging /biomedical imaging; biomedical facility; brain injury; cerebral ischemia /hypoxia; laboratory rat; lactates; magnesium; magnetic resonance imaging; method development; nuclear magnetic resonance spectroscopy; perinatal

The overall objective of this Core is to develop and apply multi-nuclear magnetic resonance (NMR) spectroscopy and imaging core facilities for the study of hypoxic-ischemic brain damage in developing rats. The spectroscopy component of the Core will apply MR spectroscopy techniques to evaluate metabolic alterations which occur during and following cerebral hypoxia-ischemia in developing rats. Spatially localized 31P and 1H MR spectroscopy will be used to quantitate changes in energy metabolism, lactate concentrations, intracellular pH, and free cytosolic magnesium concentrations. The imaging component of the Core will continue to develop and apply magnetic resonance imaging (MRI) techniques to evaluate perinatal hypoxic-ischemic brain damage. Two imaging techniques will continue tobe used to elucidate the sites and severities of brain damage at different stages of hypoxia-ischemia and recovery. These techniques include diffusion and T2-weighted spin echo imaging. Our previous results have indicated that diffusion imaging is a sensitive method for early detection of hypoxic-ischemic injury, while T2-weighted spin echo imaging more accurately monitors long-term damage. Animal movement and multi- animal devices essential for obtaining reliable images will be continually designed, constructed and refined. These devices will be incorporated into the general probe design for spectroscopy and imaging.

该核心的总体目的是开发和应用多核磁共振（NMR）光谱和成像核心设施，以研究发育中的大鼠缺氧 - 缺血性脑损伤。核心的光谱成分将应用MR光谱技术来评估发育中大鼠脑缺氧 - 缺血性缺血期间和之后发生的代谢改变。空间定位的31p和1H MR光谱法将用于定量能量代谢，乳酸浓度，细胞内pH和游离胞质镁浓度的变化。核心的成像成分将继续开发和应用磁共振成像（MRI）技术来评估围产期缺氧 - 缺血性脑损伤。两种成像技术将继续用于阐明在低氧 - 缺血和恢复阶段的脑损伤部位和严重性。这些技术包括扩散和T2加权自旋回波成像。我们先前的结果表明，扩散成像是早期检测缺氧性损伤的灵敏方法，而T2加权自旋回波更准确地监视长期损害。动物运动和对获得可靠图像必不可少的动物设备将不断设计，构造和精制。这些设备将纳入用于光谱和成像的一般探针设计中。