CAPILLARY TECHNOLOGY

毛细管技术

• 批准号: 6241418
• 负责人: HAROLD P SWERDLOW
• 金额: $ 52.82万
• 依托单位: UNIVERSITY OF UTAH
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-04-01 至 1998-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6241418
• 关键词: biomedical equipment development; biomedical facility; biotechnology; capillary electrophoresis; computer assisted sequence analysis; genome; high performance liquid chromatography; nucleic acid sequence; polymerase chain reaction

The Capillary Technology group (Project 5) will explore the application of capillary electrophoresis and microfluidics to DNA sequencing and DNA based diagnostics. A prototype diagnostic instrument has been constructed which is capable of performing PCR amplifications in a small diameter tube, purifying the reactions and efficiently loading the products onto a capillary electrophoresis instrument for analysis. The entire procedure is automated in a simple fashion. The complete reaction, purification and analysis is complete in under 30 minutes. The extension of this system to DNA sequencing is the primary goal of this proposal. Applications in genotyping using microsatellite repeat polymorphisms and PCR diagnostics will also be pursued. Improvements to the basic instrument will be made in the areas of throughput, sensitivity, economy and utility. A multi (TM) capillary version of the instrument will be constructed in the latter part of the grant. Our group will also continue to examine the applicability of the technology we developed for reloading capillary gels to slab gel systems. We will work to reduce the effort and cost of our present methods, while increasing the number of times such gels can be used beyond our current limit of four consecutive runs.

毛细管技术集团（项目5）将探讨 毛细血管电泳和微流体对DNA测序和DNA的基于DNA 诊断。 已经建造了一种原型诊断仪器 能够在小直径管中进行PCR扩增， 净化反应并有效地将产品加载到A上 毛细管电泳仪器进行分析。 整个过程是 以简单的方式自动化。 完整的反应，纯化和 分析在30分钟内完成。 该系统扩展到 DNA测序是该提案的主要目标。 应用程序 使用微卫星重复多态性和PCR诊断的基因分型 也将被追捕。 基本工具的改进将在 吞吐量，灵敏度，经济和效用领域。 多（TM） 该乐器的毛细管版将在后部构建 赠款。 我们的小组还将继续检查 我们为重新加载毛细管凝胶制定的技术来固定凝胶 系统。 我们将努力减少现在的努力和成本 方法，同时可以使用此类凝胶的次数 我们目前连续四次运行的限制。