CAPILLARY TECHNOLOGY

毛细管技术

• 批准号: 3735770
• 负责人: HAROLD P SWERDLOW
• 金额: --
• 依托单位: UNIVERSITY OF UTAH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3735770
• 关键词: biomedical equipment development; biomedical facility; biotechnology; computer assisted sequence analysis; gel electrophoresis; genome; high performance liquid chromatography; nucleic acid sequence; polymerase chain reaction

The Capillary Technology group (Project 5) will explore the application of capillary electrophoresis and microfluidics to DNA sequencing and DNA based diagnostics. A prototype diagnostic instrument has been constructed which is capable of performing PCR amplifications in a small diameter tube, purifying the reactions and efficiently loading the products onto a capillary electrophoresis instrument for analysis. The entire procedure is automated in a simple fashion. The complete reaction, purification and analysis is complete in under 30 minutes. The extension of this system to DNA sequencing is the primary goal of this proposal. Applications in genotyping using microsatellite repeat polymorphisms and PCR diagnostics will also be pursued. Improvements to the basic instrument will be made in the areas of throughput, sensitivity, economy and utility. A multi (TM) capillary version of the instrument will be constructed in the latter part of the grant. Our group will also continue to examine the applicability of the technology we developed for reloading capillary gels to slab gel systems. We will work to reduce the effort and cost of our present methods, while increasing the number of times such gels can be used beyond our current limit of four consecutive runs.

毛细管技术小组（项目5）将探索应用 毛细管电泳和微流体技术到 DNA 测序和 DNA 基础 诊断。 已经构建了原型诊断仪器 能够在小直径管中进行 PCR 扩增， 纯化反应并有效地将产物装载到 毛细管电泳仪进行分析。 整个过程是 以简单的方式实现自动化。 完成反应、纯化和 分析在 30 分钟内完成。 将该系统扩展至 DNA 测序是该提案的主要目标。 应用领域 使用微卫星重复多态性和 PCR 诊断进行基因分型 也将被追究。 基本仪器的改进将在 吞吐量、灵敏度、经济性和实用性等领域。 多 (TM) 毛细管版本的仪器将在后期建造 的补助金。 我们的小组还将继续研究其适用性 我们开发的用于将毛细管凝胶重新加载到平板凝胶的技术 系统。 我们将努力减少目前的工作量和成本 方法，同时增加此类凝胶的使用次数 我们目前的连续四次运行限制。