INTEGRATED MICROINJECTOR AND IMAGE PROCESSING SYSTEM

集成显微注射器和图像处理系统

• 批准号: 2593156
• 负责人: ANNA MARIE SKALKA
• 金额: $ 13.8万
• 依托单位: RESEARCH INST OF FOX CHASE CAN CTR
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-08-15 至 1998-08-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2593156
• 关键词: biomedical equipment purchase; image processing

This application requests funding for an integrated system that incorporates the following components: a micromanipulation- microinjection apparatus, and inverted microscope with phase contrast, epi-fluorescence and Namarski DIC optics and a CCD camera with an image processing system. At present, there is neither a system of comparable capability at Fox Chase Cancer (FCCC) nor is there available funding for such instrumentation. The research interests of the 11 NIH funded investigators who will be the major users of the requested instrumentation vary widely. However, their research efforts all converge in the detailed analysis of cultured cells at the microscopic level. Acquisition of the requested system will permit an extension of each investigator research from analysis of cell populations to the level of single cells. The various components of the requested system were chosen based on the diversity of this research interest, with the goal of satisfying the investigators~ immediate, as well as long term needs. The ultimate goal of all of the research programs described in this application is to understand human disease processes. This will be accomplished through study of the regulation of cell morphology and growth by tumor suppressors, oncogenes, signal transduction molecules, viruses and modifiers of cell polarity. Many of the cellular effectors which will be investigated in these studies have already been identified through analysis of mutations in a variety of malignant states in humans. Some of the proposed experiments describe analysis of hepatitis delta and hepatitis B viral replication, which may ultimately aid in the treatment of hepatitis, a disease that is associated with significant morbidity in humans and represents the leading cause of hepatocellular carcinoma. Others involve analysis of retroviral systems of relevance to cancer and AIDS. All of these projects share a common need for high-resolution optics, micromanipulation and microscope image processing capabilities, a requirement that can be served most effectively by access to the requested system. The requested system will be housed, operated and technically supported in the Cell Culture Facility a FCCC. The shared Facility is an ideal location for the integrated system as it serves the majority of NIH funded laboratories at FCCC and is a hub of collaborative efforts with outstanding, highly trained technical support.

该应用程序要求为一个集成系统提供资金，该系统 包含以下组成部分：微观计算 - 显微注射设备和相位显微镜 对比度，荧光和Namarski DIC光学和CCD摄像头 使用图像处理系统。 目前，没有 Fox Chase Cancer（FCCC）的可比能力系统也不是 有可用于此类仪器的资金。 研究 NIH资助的11位研究人员的利益，将成为主要 请求的仪器的用户差异很大。 但是，他们 研究工作都融合了对文化的详细分析 细胞在微观水平上。 收购请求的系统 将允许从分析扩展每个研究者研究 细胞群体达到单细胞水平。 各种 根据多样性选择了请求系统的组件 这项研究兴趣，目的是满足 调查人员〜立即以及长期需求。 在此描述的所有研究计划的最终目标 应用是了解人类疾病过程。 这将是 通过研究细胞形态的调节和 肿瘤抑制剂，癌基因，信号转导生长 细胞极性的分子，病毒和修饰剂。 许多 在这些研究中将研究的细胞效应子已 已经通过分析多种突变来确定 人类的恶性国家。 一些提出的实验 描述乙型肝炎和丙型肝炎病毒的分析 复制，最终可能有助于治疗肝炎， 与人类发病率显着的疾病， 代表肝细胞癌的主要原因。 其他的 涉及分析与癌症相关的逆转录病毒系统和 艾滋病。 所有这些项目都有对高分辨率的共同需求 光学，显微镜和显微镜图像处理 功能，这一要求最有效地满足的要求 访问请求的系统。 所请求的系统将被容纳，操作并在技术上进行 在细胞培养设施中支持FCCC。 共享设施 是集成系统的理想位置 FCCC的大多数NIH资助实验室，是 与杰出的，受过良好训练的技术的合作努力 支持。