PERSEPTIVE BIOSYSTEMS MALDI-TOF MASS SPECTROMETER

透视生物系统 MALDI-TOF 质谱仪

• 批准号: 2791805
• 负责人: GREGORY A GRANT
• 金额: $ 24.96万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-04-01 至 2000-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2791805
• 关键词: analytical chemistry; biomedical equipment; biomedical equipment purchase; matrix assisted laser desorption ionization

The goal of this application is to enhance the mass spectrometry capabilities available to NIH investigators at Washington University School of Medicine and in so doing significantly strengthen the potential of those investigators to conduct outstanding biomedical research. This will be accomplished by the purchase of a modern Perseptive Biosystems MALDI-TOF mass spectrometer to complement the Finnigan LCQ ESI instrument already in operation. This instrument will enhance the capabilities of NIH supported investigators at Washington University by providing increased sensitivity, versatility of chemistry, and cost-effectiveness in mass spectrometry services. The instrument will be integrated into a Core Facility and be operated by the Washington University Protein and Nucleic Acid Chemistry Laboratories as a shared instrument for biomedical research. A major group of 6 NIH supported investigators has been identified as primary users whose research will be enhanced immediately by acquisition of the instrument. These investigators are funded by 5 different institutes of NIH and require the instrument for projects that include biochemical, biophysical, and structure-function studies in coagulation, the role of inositol phosphates in human disease, enzymatic mechanism of allostery, pili assembly in bacterial pathogenesis, RNA binding function, and gene expression in bone. In addition, the instrument will be utilized by an additional group of approximately 52 Acid Chemistry Laboratories. Finally, approximately 80 additional NIH funded investigators who use the Nucleic Acid Chemistry Laboratories have operated as a core service facility for over 15 years. Thus, all of the necessary requirements for scientific expertise, operation, maintenance, and cost-recovery are in place. In addition, the staff of the Protein and Nucleic Acid Chemistry Laboratories have over 5 years of experience in mass spectrometry analysis.

该应用的目的是增强华盛顿大学医学院的NIH调查人员可用的质谱能力，从而大大增强了这些研究人员进行杰出生物医学研究的潜力。这将通过购买现代的感官生物系统MALDI-TOF质谱仪来完成，以补充已经在运行的Finnigan LCQ ESI仪器。该仪器将通过提高质谱服务的敏感性，多功能性和成本效益，从而提高华盛顿大学NIH支持研究人员的能力。该仪器将被整合到核心设施中，并由华盛顿大学的蛋白质和核酸化学实验室作为生物医学研究的共享工具进行操作。已将6名NIH支持调查人员组成的主要组被确定为主要用户，他们的研究将通过获取仪器立即增强。这些研究者由NIH的5个不同机构提供资金，并要求该仪器用于包括生化，生物物理和结构功能研究在凝结中，肌醇磷酸盐在人体疾病中的作用，酶变构的作用，pili的酶促机制，PILI组装在细菌病原体中，RNA的结合功能以及骨骼中的基因表达。此外，该仪器将由大约52个酸化实验室的其他组使用。最后，使用核酸化学实验室的大约80名NIH资助的研究人员已作为核心服务设施运作了15年以上。因此，对科学专业知识，运营，维护和成本恢复的所有必要要求都存在。此外，蛋白质和核酸化学实验室的工作人员在质谱分析方面具有超过5年的经验。