CORE--AMINO ACID FACILITY

核心——氨基酸设施

• 批准号: 6414871
• 负责人: PAUL J FLAKOLL
• 金额: $ 21.13万
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-12-01 至 2002-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6414871
• 关键词: alanine; aminoacid; analytical method; biomedical equipment purchase; biomedical facility; blood chemistry; branched chain aminoacid; cerebrospinal fluid; diabetes mellitus; high performance liquid chromatography; ion exchange chromatography; leucine; phenylalanine; protein sequence; protein structure; urinalysis

The Amino Acid Assay Subcore provides investigators access to a number of analytical methods related to the measurement of labelled and unlabelled amino acids. The subcore provides space, equipment and personnel for sample analysis and method development. Investigators pay a charge to cover the cost of supplies and pro-rated service contracts. The analyses available include 1) complete amino acid profiles, 2) select amino acid profiles (branched chain or gluconeogenic), 3) amino acid specific activity determinations (alanine, leucine, phenylalanine) and 4) amino acid enrichment (leucine, alpha ketoisocaproate, phenylalanine). With the exception of the rapid assay for leucine and phenylalanine, the user group identified as being associated with this subcore project an increased need for all services offered.

氨基酸测定子核使研究人员访问了许多 与标记和未标记的测量有关的分析方法 氨基酸。该子核心为空间，设备和人员提供 样本分析和方法开发。调查人员向 支付供应成本和评分服务合同。分析 可用包括1）完整的氨基酸剖面，2）选择氨基酸 轮廓（分支链或糖原），3）特异性氨基酸特异性 活性测定（丙氨酸，亮氨酸，苯丙氨酸）和4）氨基 酸富集（亮氨酸，α酮二木酸酯，苯丙氨酸）。与 用户组的亮氨酸和苯丙氨酸的快速测定除外 确定与该子核项目相关联 对于提供的所有服务。