CORE--NEUROPATHOLOGY FACILITY

核心——神经病理学设施

• 批准号: 6098281
• 负责人: ELIZABETH J. COCHRAN
• 金额: $ 15.71万
• 依托单位: RUSH UNIVERSITY MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-05-01 至 2000-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6098281
• 关键词: Alzheimer's disease; biomedical facility; biopsy; brain; brain disorder diagnosis; histopathology; human tissue; postmortem; tissue resource /registry

The neuropathology Core will provide, to the researchers of the Program Project, brain tissue and diagnostic neuropathology evaluations on Alzheimer's disease (AD) patients and controls, from subjects who have been closely followed in the Clinical Core with neurological and neuropsychological examinations. Brain tissue with minimal post-mortem intervals will be obtained and preserved so as to fill the needs of the three Program Project investigators. The abundant brain tissue received by the Rush Brain Bank from the Rush Alzheimer's Disease Center (RADC) and the establishment of new sources of control tissue, the Religious Order Study (ROS) and the RADC control program will allow the Neuropathology Core to fulfill the needs of the investigators. Each project will study a series of clinically defined brains': 8 controls without dementia and 24 AD patients divided equally between three groups; mild, moderate and severe cognitive impairment. One project will use fixed basal forebrain tissue and snap frozen cortical tissue, for immunohistochemical and in situ hybridization studies. Other projects will use fixed and snap frozen basal forebrain and temporal lobe tissue for immunohistochemistry, in situ hybridization, receptor binding and radioimmunoassay studies. The RADC will be the primary source of the moderately and severely impaired AD patients, and the RADC control study ad the Religious Order Study will be the source of the mildly impaired AD patients and control subjects. A thorough neuropathological evaluation will be performed on each case using standard diagnostic criteria for Alzheimer's disease (NIH consensus criteria), Parkinson's disease (CERAD), and related disorders. A direct entry data program will be used for all gross and microscopic neuropathological information. With the use of a specimen tracking software program (FreezerWorks), all tissue will be accurately indexed allowing rapid and reliable distribution of tissue to investigators. Close linkage will be maintained with the clinical core through monthly meetings to facilitate tissue distribution. The brain tissue and neuropathological data provided to investigators by the Neuropathology Core is critical for the success of the projects and for the provision of seminal information about nerve growth factor trophism, galanin remodeling, and altered tau conformation and the progression of Alzheimer's disease.

神经病理学核心将为该计划的研究人员提供 项目，脑组织和诊断性神经病理学评估 阿尔茨海默氏病（AD）患者和对照组，来自曾经的受试者 在神经系统和 神经心理学检查。 脑组织具有最小的验尸 将获得并保留间隔，以满足 三个计划项目调查员。 由 阿尔茨海默氏病中心（RADC）的Rush Brain Bank和 建立新的控制组织来源，宗教秩序研究 （ROS）和RADC控制程序将允许神经病理学核心 满足调查人员的需求。 每个项目都会研究一个系列 临床定义的大脑：8个无痴呆症的控制和24 AD 患者平均分配三组；轻度，中度和重度 认知障碍。 一个项目将使用固定的基础前脑组织 并快照冷冻的皮质组织，用于免疫组织化学和原位 杂交研究。 其他项目将使用固定并捕捉冻结的基础 原位的前脑和颞叶组织进行免疫组织化学 杂交，受体结合和放射免疫测定研究。 radc 将是中等和严重受损的广告的主要来源 患者和RADC对照研究AD宗教秩序研究将是 轻度受损的AD患者和对照组的来源。 一个 使用 阿尔茨海默氏病的标准诊断标准（NIH共识 标准），帕金森氏病（CERAD）和相关疾病。 直接 输入数据程序将用于所有总体和显微镜 神经病理学信息。 使用标本跟踪 软件程序（冰柜），所有组织都将被准确索引 允许组织对研究人员的快速分布。 关闭 连接将通过每月会议与临床核心保持 促进组织分布。 脑组织和神经病理学数据由 神经病理学核心对于项目的成功至关重要 提供有关神经生长因子营养不良的精确信息， 加拉蛋白重塑，改变了tau构象和进展 阿尔茨海默氏病。