DETERMINANTS OF SYNAPTIC STRENGTH IN MUSCLE

肌肉突触强度的决定因素

• 批准号: 6165413
• 负责人: ROBERT S WILKINSON
• 金额: $ 18.87万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-04-01 至 2001-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6165413
• 关键词: action potentials; biological models; electron microscopy; electrophysiology; fluorescent dye /probe; immunologic assay /test; microelectrodes; neural plasticity; neuromuscular junction; neurophysiology; neurotransmitter transport; snakes; sodium channel; striated muscles; synapses; synaptic vesicles

The proposed project examines the determinants of synaptic strength in the vertebrate nervous system, using the neuromuscular junction as a model. The thin muscle used (snake transversus abdominis) is highly stereotyped and contains a variety of types of motor synapses, all accessible for microscopic and electrophysiological examination. A recent experimental technique, called synaptic reconstruction, is utilized. The technique permits physiological study of neurotransmitter release from one synaptic bouton, the smallest anatomical component of a chemical synapse. As a result, the present need for statistical inference to characterize one bouton's behavior based on that of a population is obviated. in addition, anatomical methods have been developed to visualize structures associated with processing and release of transmitter within the bouton, and structures associated with transmitter-induced conductance change within the postsynaptic receptor membrane. The project's goal is to learn what pr and postsynaptic structures determine the strength of a synapse, and what modifications of these structures are associated with the regulation, or plasticity, of synaptic strength in adults. This knowledge is essential in understanding why such regulation fails in diseases which attack pre-or postsynaptic cells throughout the nervous system.

拟议的项目检查了突触强度的决定因素 脊椎动物神经系统，使用神经肌肉接头作为模型。 使用的薄肌肉（蛇腹横肌）是高度定型的 并包含多种类型的运动突触，所有这些都可用于 显微镜和电生理检查。 最近的一个实验 使用称为突触重建的技术。 技术 允许对一个突触释放的神经递质进行生理学研究 bouton，化学突触的最小解剖成分。 作为一个 结果，目前需要统计推断来表征一个 布顿基于总体行为的行为被消除。 此外， 已经开发出解剖方法来可视化相关结构 在按钮内处理和释放发射机，以及 与发射器引起的电导变化相关的结构 突触后受体膜。 该项目的目标是了解什么是 pr 突触和突触后结构决定了突触的强度，以及什么 这些结构的修改与法规相关，或 可塑性，成人突触强度。 这些知识对于 了解为什么这种调节在攻击前或前的疾病中失败 整个神经系统的突触后细胞。