MOLECULAR CHARACTERIZATION OF NEURAL CREST INDUCTION

神经嵴诱导的分子表征

• 批准号: 6208946
• 负责人: LAURA S GAMMILL
• 金额: $ 2.36万
• 依托单位: CALIFORNIA INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-01-08 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6208946
• 关键词: developmental genetics; developmental neurobiology; gene expression; genetic library; in situ hybridization; neural crest; nucleic acid sequence

Neural crest cells are progenitors of diverse and numerous lineages in the vertebrate embryo, including the peripheral nervous system and the bones and cartilage of the head. Neural crest cells arise in the neural folds as the neurectoderm rolls into a neural tube, then migrate to a variety of distant sites in the periphery of the embryo. Despite the fundamental importance of the neural crest for vertebrate development, it is not known how these cells form. The goal of this proposal is to define the molecular events that generate neural crest cells. An arrayed cDNA library will be created from a pool of embryos at different stages of neural crest development. Neural crest-specific genes on the array will be identified by hybridizing subtracted, labeled cDNA made from tissues that are induced versus uninduced for neural crest. From this pool, regulators of neural crest cell identity will be identified by three criteria: l) sequence, 2) ability to induce neural crest when ectopically expressed by electroporation, and 3) expression pattern. In future work, genes identified by this technique will be functionally characterized in order to understand the cascade of events that gives rise to neural crest cells.

神经rest细胞是脊椎动物胚胎中多种谱系的祖细胞，包括周围神经系统以及头部的骨骼和软骨。神经rest细胞出现在神经褶皱中，因为神经外胚层将其滚入神经管，然后迁移到胚胎周围的各种远处。尽管神经rest对于脊椎动物发育至关重要，但尚不清楚这些细胞如何形成。该建议的目的是定义产生神经rest细胞的分子事件。在神经rest发育的不同阶段，将从胚胎池创建一个阵列的cDNA库。阵列上的神经克雷斯特特异性基因将通过减去由诱导的组织而不是诱导的神经rest的组织制成的杂交，标记的cDNA来识别。从该池中，将通过三个标准来识别神经rest细胞身份的调节剂：l）序列，2）当异位通过电穿孔表达时诱导神经rest的能力和3）表达模式。在将来的工作中，该技术确定的基因将在功能上表征，以了解产生神经rest细胞的事件的级联。