SPATIOTEMPORAL REGULATION OF FIBRONECTIN

纤连蛋白的时空调控

• 批准号: 6138853
• 负责人: Stuart A. Newman
• 金额: $ 7.42万
• 依托单位: NEW YORK MEDICAL COLLEGE
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-01-01 至 2000-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6138853
• 关键词: animal genetic material tag; fibroblast growth factor; fibronectins; gene expression; histogenesis; limbs; protein biosynthesis; retinoids; tissue /cell culture; transcription factor; transforming growth factors

DESCRIPTION (Adapted from applicant's description): The goals of this project are to test the hypothesis that skeletal pattern formation in the vertebrate limb arises, in part, from the intrinsic, self-organizing properties of limb bud precartilage mesenchymal tissue. The focus of the studies will be on the regulatory mechanisms of fibronectin's spatiotemporal expression in limb mesenchyme in vitro and in vivo. Specific aims are: 1) to identify the sequences of the fibronectin promoter that are involved in the spatiotemporal expression of fibronectin during limb mesenchymal condensation and to delineate candidate sites for the binding of developmentally relevant transcription factors; and 2) to test the hypothesis that endogenous factors of the developing limb such as TGF-beta, FGF-2 and -4, and retinoic acid, regulate chondrogenic pattern formation via fibronectin gene expression.

描述（根据申请人的描述改编）： 该项目的目标是检验骨骼的假设 脊椎动物肢体中的图案形成部分是由 肢体芽前胎的固有的自组织特性 间充质组织。 研究的重点将放在监管上 纤连蛋白在肢体间充质中时空表达的机理 体外和体内。 具体目的是：1）识别序列 纤连蛋白启动子的时空启动子 肢体间充质凝结期间纤连蛋白的表达和至 描述候选站点的结合 转录因子； 2）检验内源性的假设 发育中的肢体的因素，例如tgf-beta，fgf-2和-4，以及 视黄酸，通过纤连蛋白调节软骨形成的形成 基因表达。