HISTONE LIKE PROTEINS ASSOCIATED WITH KINETOPLAST DNA

与动质体 DNA 相关的组蛋白样蛋白

• 批准号: 6170371
• 负责人: DAN S RAY
• 金额: $ 21.22万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-08-01 至 2003-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6170371
• 关键词: Crithidia; DNA binding protein; DNA footprinting; DNA replication; chemical binding; circular DNA; eukaryote; gel filtration chromatography; histones; immunoprecipitation; mitochondrial DNA; polymerase chain reaction; protein structure function; sedimentation; western blottings

DESCRIPTION (Adapted from the Applicant's Abstract): Kinetoplastid protozoa are the causative agents of visceral and cutaneous leishmaniasis, sleeping sickness and Chagas' disease in much of the tropical and subtropical world. These parasitic organisms are characterized by a unique form of mitochondrial DNA called a kineplast DNA or kDNA. This DNA consists of several thousand minicircles and about 25 maxicircles, all of which are interlocked into a single DNA network which is condensed into a highly organized disk-shaped structure at the base of the flagellum. Circular DNA molecules are released individually from the network, replicated and then rejoined to the network which eventually doubles in size as all circular DNA molecules become duplicated. The double sized network is then divided into two and a united size network is partitioned to each of two daughter cells prior to completion of cell division. This research will investigate the role of the group of histone-like kinetoplast proteins in the organization and segregation of the kDNA in the kinetoplastid Crithidia fasciculata.

描述（改编自申请人的摘要）：动型原生动物是 内脏和皮肤利什曼病的病因，昏昏欲睡的疾病 以及在热带和亚热带世界中的大部分木马疾病。这些 寄生生物的特征是一种独特的线粒体DNA 称为动型塑料DNA或kDNA。该DNA由数千个 微圆和大约25个最大环，所有这些都互锁到一个 单个DNA网络被凝结成高度有条理的磁盘形 鞭毛底部的结构。圆形DNA分子被释放 单独从网络中复制，然后重新加入网络 随着所有圆形DNA分子变为 重复。然后将双尺寸网络分为两个和一个统一的大小 在完成之前，将网络分配到两个子细胞中的每个细胞中的每个细胞 细胞分裂。这项研究将调查该小组的作用 组织中类似组蛋白的动力学蛋白在组织中和隔离 kDNA在动力学crithidia fasciculata中。