CELLULAR IMMUNITY TO CRYPTOSPORIDIUM PARVUM

对小隐孢子虫的细胞免疫

• 批准号: 6169792
• 负责人: Anthony R. Hayward
• 金额: $ 14.72万
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-07-01 至 2001-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6169792
• 关键词: BCL2 gene /protein; CD40 molecule; Cryptosporidium; T lymphocyte; biliary tract; biliary tract disorder; cellular immunity; cryptococcosis; cytokine receptors; gene expression; genetically modified animals; interferon gamma; intestines; laboratory mouse; ligands; microorganism immunology

DESCRIPTION (Adapted from the Applicant's Abstract): The investigator proposes to test the hypothesis that binding of CD40L on activated T cells to CD40+ on infected epithelial cells contributes to immunity against Cryptosporidium parvum (Cp) in the biliary tree and the intestine. The investigator proposes to investigate the mechanisms by which CD40-CD40L interactions contribute to Cp immunity by determining whether: 1. CD40L is required for the afferent or efferent limb of Cp specific immunity by transfers of Cp immune and non-immune cells into Cp infected MHC matched knockout recipients. 2. The intact genes for gamma-IFN, TNFR, and/or IL 12 are required for sclerosis of bile ducts in Cp infected mice. 3. The synthesis of Bcl-series proteins is inhibited on a cell-specific basis by Cp infection. If the investigator shows that Cp can be eliminated from the biliary tree by non-specific as well as specific T-cell mediated responses, a subsequent clinical study in AIDS patients, using discriminant analysis, would be planned.

描述（改编自申请人的摘要）：调查员 提出测试CD40L与活化T细胞结合的假设 对感染上皮细胞的CD40+有助于免疫 胆汁树和肠道中的隐孢子虫（CP）。 这 研究人员建议研究CD40-CD40L的机制 通过确定是否：1。CD40L，相互作用有助于CP免疫力 是CP特异性免疫的传入或传入的肢体所必需的 将CP免疫和非免疫细胞转移到CP感染的MHC中 敲除接收者。 2。γ-IFN，TNFR和/或IL的完整基因 在CP感染小鼠中胆管硬化需要12。 3 BCl系列蛋白的合成是通过细胞特异性抑制的 CP感染。 如果调查员表明可以从中消除CP 非特异性和特定T细胞介导的胆道树 反应，随后在AIDS患者中进行的临床研究，使用 判别分析将被计划。