CORE--PRIMATE STEM CELL TRANSPLANTATION

核心——灵长类干细胞移植

• 批准号: 6365281
• 负责人: ROBERT GOFF ANDREWS
• 金额: $ 25.65万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-28 至 2005-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6365281
• 关键词: Macaca nemestrina; baboons; biomedical facility; biotechnology; cooperative study; gene therapy; hematopoietic stem cells; stem cell transplantation

Primate stem transplantation core. The Primate Stem Cell Transplantation Core will provide three major functions for the investigators of the UW/FHCRC PEGT and for NHLBI investigators. 1) The Core will provide hematopoietic progenitor and stem cell populations from non- human primates, baboons (Papio cynocephalus sp.) and pig-tailed macaques (Macaca nemistrina), for in vitro gene transfer experiments. 2) It will undertake the conduct of in vivo transplantation experiments with gene modified marrow-repopulating cells purified from mobilized blood and/or primed marrow. 3) It will undertake the conduct of studies to test noel tem/progenitor cell mobilization schemes to be used for stem cell gene therapy. Access to large animal transplant models for hematopoietic stem cell gene therapy can be a limiting factor when investigators try to extrapolate findings form the mouse and other small animal models to the human clinical setting. The Primate Stem Cell Transplant Core will function to address these limitations. The Core will be available to all PET supported investigators as well as other NHLBI researchers. In collaboration with the Cell Procurement and Processing Core, specific blood and marrow populations from baboons and macaque species will be provided to investigators for in vitro studies. Importantly, the Core will take advantage of activities in stem cell transplantation and gene transfer in baboons that already exist at the UW-Regional Primate Research Center and FHCRC. The Core not only will provide the care and management of transplanted animals but also will provide expertise on in vitro studies of hematopoiesis, performing transductions, analyzing cells from animals post-transplant and protocol development. To optimize the use of available animals, competitive repopulating strategies will be used to allow direct intra-animal comparisons of different protocols and vectors. The ability of this Core to provide collaborating investigators with a relevant large animal model should greatly facilitate advances in stem cell biology and the eventual application of hematopoietic stem cell gene therapy to the clinical setting.

灵长类动物茎移植核心。灵长类动物干细胞移植核心将为UW/FHCRC PEGT和NHLBI研究者的研究人员提供三个主要功能。 1）核心将提供来自非人类灵长类动物的造血祖细胞和干细胞种群，狒狒（Papio cynocephalus sp。）和猪尾猕猴（Macaca nemistrina）用于体外基因转移实验。 2）它将通过基因修饰的骨髓重塑细胞进行体内移植实验的进行，从动员的血液和/或启动骨髓进行纯化。 3）它将进行研究，以测试用于干细胞基因治疗的NOEL TEM/祖细胞动员方案。当研究人员试图推断出小鼠和其他小动物模型到人类的临床环境时，访问大型动物移植模型进行造血干细胞基因疗法可能是一个限制因素。灵长类动物干细胞移植核心将起作用以解决这些局限性。该核心将提供给所有宠物支持的研究人员以及其他NHLBI研究人员。与细胞采购和加工核心合作，将向研究人员提供来自狒狒和猕猴的特定血液和骨髓种群进行体外研究。重要的是，核心将利用在UW区域灵长类动物研究中心和FHCRC中已经存在的狒狒中的干细胞移植和基因转移活性。核心不仅将提供移植动物的护理和管理，而且还将提供有关造血的体外研究，进行转导，分析移植后动物的细胞和方案开发的细胞的专业知识。为了优化可用动物的使用，将使用竞争性重新流传策略来直接对不同方案和向量的动物内比较。该核心为合作研究者提供相关大型动物模型的能力应极大地促进干细胞生物学的进步，并最终将造血干细胞基因治疗应用于临床环境。