MICROFILAMENTS IN THE YEAST SACCHAROMYCES CEREVISIAE

酿酒酵母中的微丝

• 批准号: 6179548
• 负责人: Anthony P. Bretscher
• 金额: $ 26.21万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-02-01 至 2002-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6179548
• 关键词: Golgi apparatus; Saccharomyces cerevisiae; actins; cellular polarity; fungal genetics; gene expression; gene mutation; immunoelectron microscopy; immunofluorescence technique; microfilaments; molecular cloning; molecular genetics; protein structure function; protein transport; secretory protein; tropomyosin

The goal of the proposed studies is to understand the molecular details responsible for the transport and targeting of secretory products via the actin cytoskeleton. Current results suggest that secretory vesicles are transported down actin cables by a myosin V. The proposed experiments explore how transport is affected in mutants with altered polarity and what features of the actin cytoskeleton are required to allow it to function as a myosin V substrate. Four specific aims are presented: (1) to develop a system for the localization of the sites of emergence of secretory products; (2) to produce temperature sensitive tropomyosin (tpm1) mutants that will be used to investigate polarization and depolarization of the actin cytoskeleton and secretory processes; (3) to screen for mutant that have a tpm1 sensitive secretory defect; and (4) to identify proteins that interact with the myosin V tail by biochemical and genetic approaches.

拟议研究的目的是了解分子细节 负责通过 肌动蛋白细胞骨架。 当前的结果表明分泌囊泡 由肌球蛋白V沿肌动蛋白电缆运输。 实验探讨了在变化的突变体中如何影响运输 极性以及肌动蛋白细胞骨架的哪些特征才能 允许其充当肌球蛋白V底物。 四个具体目标是 介绍：（1）开发一个用于本网站本地化的系统 分泌产品的出现； （2）产生温度敏感的 tropomyosin（TPM1）突变体，用于研究极化 以及肌动蛋白细胞骨架和分泌过程的去极化； （3）筛选具有TPM1敏感分泌缺陷的突变体； （4）通过通过 生化和遗传方法。