PHOTOBIOLOGY OF RHODOPSIN, BACTERIORHODOPSIN & VIODOPSIN

视紫红质、细菌视紫红质的光生物学

• 批准号: 6164773
• 负责人: ROBERT Richards BIRGE
• 金额: $ 19.55万
• 依托单位: UNIVERSITY OF CONNECTICUT STORRS
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-08-01 至 2002-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6164773
• 关键词: Xenopus; analog; bacteriorhodopsins; biophysics; calorimetry; cations; chemical substitution; chromophore; infrared spectrometry; interferometry; microwave spectrometry; molecular dynamics; molecular site; photochemistry; photoelectron spectrometry; protein structure; quantum chemistry; rhodopsin; site directed mutagenesis; visual pigments

DESCRIPTION: The nature of the chromophore binding sites and the primary photochemical events of rhodopsin, bacteriorhodopsin and the Xenopus violet cone pigment, viodopsin, will be studied by using spectroscopic and theoretical techniques. The goals are to understand the photophysical properties of the bound chromophores. The principal spectroscopic methods to be used in these studies include two-photon spectroscopy, Stark effect spectroscopy, Fourier transform infra-red spectroscopy, microwave spectroscopy, and pulsed laser photocalorimetry. The principal chemical studies to be undertaken include organic cation and chromophore analog substitutions, as well as site directed mutagenesis. The theoretical methods include semiempirical molecular orbital theory and molecular dynamics theory. The goal is to combine experiment and theory in a synergistic program which enhances both. In addition to the more global goals outlined above, Dr. Birge will seek to answer the following specific questions: (1) What is the principal mechanism of wavelength modulation in the blue and violet cones? (2) Where are the cation binding sites in bacteriorhodopsin, and how do these sites mediate the properties of the bound chromophore? (3) Is there a chloride binding site in viodopsin, and what impact does this site have on the photophysical properties of the chromophore? (4) What are the molecular origins of energy storage in the primary events of these three proteins? (5) What is the principal molecular mechanism of dark noise in vertebrate and invertebrate vision? (6) What are the molecular origins of the unusual photochemical properties of the 4-keto retinal bacteriorhodopsin analog? (7) Can one improve the accuracy of the MNDO-PSDCI semi-empirical molecular orbital theory by using ab-initio effective Hamiltonian parameterizaion?

描述：发色团结合位点和主要的性质 视紫红质，细菌紫红质和武士紫罗兰色的光化学事件 将使用光谱和 理论技术。 目标是了解光物理 结合发色团的特性。 主要光谱法 在这些研究中使用包括两光谱光谱，鲜明效应 光谱，傅立叶转化红外光谱，微波炉 光谱和脉冲激光光钙化法。 主要化学物质 进行的研究包括有机阳离子和发色团类似物 取代以及位置定向诱变。 理论 方法包括半经验分子轨道理论和分子 动力学理论。 目的是将实验和理论结合在 协同程序可以增强这两者。 除了更全球 上面概述的目标，Birge博士将寻求回答以下具体 问题：（1）波长调制的主要机制是什么 蓝色和紫罗兰？ （2）阳离子结合位点在哪里 细菌淡淡的淡淡蛋白质，这些位点如何介导 绑定的发色团？ （3）viodopsin中是否有氯化物结合位点，并且 该站点对该地点的影响 发色团？ （4）储能的分子起源是什么 这三种蛋白质的主要事件？ （5）什么是主分子 脊椎动物和无脊椎动物视觉中暗噪声的机理？ （6）是什么 4-酮的异常光化学特性的分子起源 视网膜细菌紫红素类似物？ （7）一个人可以提高 MNDO-PSDCI半经验分子轨道理论，使用AB-Initio 有效的哈密顿参数？