Photobiology of Rhodopsin & Bacteriorhodopsin

视紫红质的光生物学

• 批准号: 6643448
• 负责人: ROBERT Richards BIRGE
• 金额: $ 21.29万
• 依托单位: UNIVERSITY OF CONNECTICUT STORRS
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-08-01 至 2006-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6643448
• 关键词: Raman spectrometry; Schiff bases; analog; bacteriorhodopsins; binding sites; chemical substitution; chromophore; circular dichroism; cone cell; infrared spectrometry; interferometry; molecular site; photobiology; photochemistry; photoelectron spectrometry; protein binding; protein structure function; protonation; rhodopsin; rod cell; site directed mutagenesis; ultraviolet radiation; visual pigments

DESCRIPTION (provided by applicant): The long-term objectives of this research program are to understand the molecular details of protein photochemical mediation and wavelength regulation in bacteriorhodopsin and the visual pigments of rods and cones. Our emphasis for the present grant period is to study the blue, violet and uv cones because relatively little is known about these pigments. The key spectroscopic tools that will be used include one-photon and two-photon spectroscopy, Fourier-transform infrared spectroscopy, Raman spectroscopy, CD spectroscopy and pulsed laser photocalorimetry. The principal biochemical studies to be undertaken include site-directed mutagenesis and chromophore analog substitutions as well as random mutagenesis followed by screening for wavelength and photochemical properties. The theoretical studies will rely heavily on the use of MNDOPSDCI molecular orbital theory to probe the photophysical properties of the protein-bound chromophores. An important and new goal of this grant period is to add the prediction of circular dichroism spectra to the MNDOPSDCI procedures, with the immediate goal of using experimental CD spectra to analyze the protein binding sites of rhodopsin and the cone pigments. We will also use ab-initio and semiempirical molecularorbital theory to examine the ground state properties of the chromophore binding sites while using molecular mechanics to describe the remaining portions of the protein. Our goal is to combine experiment and theory in a synergistic program that enhances both. In addition to the more global goals outlined above, we will seek to answer the following specific questions: (1) What are the principal mechanisms of wavelength regulation in the cone pigments? (2) What is responsible for the significant difference in the absorption spectra of bacteriorhodopsin versus sensory rhodopsin II? (3) What are the mechanisms through which the chloride binding sites in the cone pigments influence the spectroscopic properties of the chromophores in the long wavelength cone pigments? (4) Where are the calcium binding sites in bacteriorhodopsin, and how do these sites influence the photophysical properties of the bound chromophore? (5) What specific proteinchromophore interactions are responsible for selecting 6-s-cis versus 6-s-trans ring conformations of the bound chromophore, and why does rhodopsin select the 6-s-cis while bacteriorhodopsin and sensory rhodopsin select the 6-s-trans conformation?

描述（由申请人提供）：本研究的长期目标 程序将了解蛋白质光化学的分子细节 细菌淡淡蛋白质和视觉的调解和波长调节 杆和锥体的颜料。我们对当前赠款期的重点是 研究蓝色，紫色和紫外线，因为对 这些颜料。将使用的关键光谱工具包括 单光子和两光子光谱，傅立叶转换红外 光谱，拉曼光谱，CD光谱和脉冲激光 光钙化法。要进行的主要生化研究包括 位置定向的诱变和发色团模拟取代以及 随机诱变，然后筛选波长和光化学 特性。理论研究将在很大程度上取决于Mndopsdci的使用 分子轨道理论探测 蛋白质结合的发色团。这个赠款期的一个重要和新的目标是 为了将圆形二色性光谱的预测添加到mndopsdci 程序，其直接目标是使用实验CD光谱分析 视紫红质和锥体色素的蛋白质结合位点。我们还将使用 Ab-Initio和半经验分子理论检查基态 发色团结合位点的特性，同时使用分子力学 描述蛋白质的其余部分。我们的目标是结合 实验和理论在协同程序中增强了这两者。此外 对于上面概述的全球目标，我们将寻求回答以下 特定问题：（1）波长的主要机制是什么 锥体色素的调节？ （2）什么是重要的 细菌紫红蛋白与感觉的吸收光谱的差异 Rhodopsin II？ （3）氯化物结合的机制是什么 锥体色素中的位点影响光谱特性 长波长锥色素中的发色团？ （4）钙在哪里 细菌视紫红质中的结合位点，这些位点如何影响 结合发色团的光物理特性？ （5）什么具体 蛋白质团子相互作用负责选择6-S-CIS与 绑定的发色团的6-S-Trans环构象，为什么Rhodopsin会 选择6-S-cis，而细菌淡淡蛋白质蛋白和感觉视蛋白选择 6-S-trans构象？