DYNAMICS OF T CELL ANTIGEN RECOGNITION PROBED W/ OPTICAL METHODS

使用光学方法探测 T 细胞抗原识别的动力学

• 批准号: 6119307
• 负责人: MICHAEL D CAHALAN
• 金额: $ 2.46万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-04-01 至 2000-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6119307
• 关键词: biological products; biomedical resource; human tissue; immunology; inflammation; lasers; minerals; model design /development

With emphasis on a single-cell approach, we are proposing experiments on antigen-specific T lymphocytes using a combination of optical techniques - confocal microscopy, two-photon microscopy, and optical tweezers - to investigate the dynamics of membrane receptor interactions during antigen presentation. Our laboratory is using biophysical techniques to investigate signaling mechanisms, biochemical and optical assays to monitor cell activation, and genetically modified cells to probe molecular mechanisms. The proposed experiments on T lymphocytes and antigen-presenting cells will focus on the dynamics of signaling from the engagement of membrane receptors to the [Ca2+]i signal. One goal of this project is to apply optical techniques to investigate antigen recognition, [Ca2+]i signaling, motility, and gene expression in T cells stimulated by physiological ligands. We will use reporter genes to visualize T-cell activation dynamically in order to relate membrane and se cond-messenger mechanisms to effector function. We are investigating interactions between cells during antigen presentation, as well as intracellular signaling mechanisms that link membrane receptors with gene expression.

强调单细胞方法，我们正在提议 结合了抗原特异性T淋巴细胞的实验 光学技术 - 共聚焦显微镜，两光子显微镜和 光学镊子 - 研究膜受体的动力学 抗原表现过程中的相互作用。 我们的实验室正在使用 生物物理技术研究信号传导机制， 生化和光学测定以监测细胞激活，以及 基因修饰的细胞以探测分子机制。 这 提出的关于T淋巴细胞和抗原呈递细胞的实验 将重点关注信号的动态 [Ca2+] i信号的膜受体。 这个项目的一个目标是 应用光学技术来研究抗原识别， [Ca2+] I信号传导，运动性和基因表达在T细胞中刺激 通过生理配体。 我们将使用记者基因可视化 动态激活T细胞激活，以将膜和SE关联 condermessenger机制具有效应子功能。 我们正在调查 抗原表现过程中细胞之间的相互作用，以及 细胞内信号传导机制将膜受体与 基因表达。