STRUCTURAL HOMOLOGIES BETWEEN KINESIN & MYOSIN SUPERFAMILIES

驱动蛋白之间的结构同源性

• 批准号: 6119263
• 负责人: KURT THORN
• 金额: $ 0.54万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-07-01 至 2000-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6119263
• 关键词: biomedical resource; enzymes; musculoskeletal system; nervous system; proteins

Kinesin and myosin are the founding members of two distinct superfamilies of proteins responsible for motility. While members of both protein families generate force and are responsible for producing motion, they show no detectable equence homology. Unexpectedly, the crystal structures of both myosin and kinesin are homologous to one another, indicating that they share a common mechanism of ATP hydrolysis and possibly a common mechanism of force generation. By comparison of the two structures, we have determined the homologous regions of the two proteins. Comparison of the homologous regions with the large numbers of known kinesin and myosin structures has enabled us to determine the crucial residues in the conserved regions. We are currently examining the possible functions of these residues in combination with molecular modeling at the Computer Graphics Laboratory. An additional goal of this project is to search for other potential motors which share the kinesin/myosin fold. We have approached this by searching Genbank for sequences which match either a fingerprint determined from the conserved regions, or which match a profile determined from the conserved regions. This work is still underway.

动力素和肌球蛋白是两个独特的创始成员 蛋白质的超家族负责运动。 而成员 两个蛋白质家族都产生力，并负责产生 运动，它们没有可检测到的均等同源性。 出乎意料的是 肌球蛋白和驱动蛋白的晶体结构与一个 另一个，表明它们具有ATP的共同机制 水解，可能是产生力的常见机制。 经过 两种结构的比较，我们确定了同源 两种蛋白质的区域。 同源区域的比较 大量已知的驱动蛋白和肌球蛋白结构具有 使我们能够确定保守区域中的关键残基。 我们目前正在研究这些残基的可能功能 在计算机图形上与分子建模的结合 实验室。 该项目的另一个目标是搜索其他 具有分享动力素/肌球蛋白折的潜在电机。 我们有 通过搜索GenBank查看匹配的序列来接近这一点 根据保守区域确定的指纹，或匹配的指纹 根据保守区域确定的轮廓。 这项工作仍然 进行。