STRUCTURAL HOMOLOGIES AMONG MOTOR PROTEINS

运动蛋白之间的结构同源性

• 批准号: 6456806
• 负责人: KURT THORN
• 金额: $ 27.32万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-07-01 至 2003-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6456806
• 关键词: Mammalia; bacteria; biomedical resource; enzymes

Conventional kinesin and myosin are founding members of two distinct superfamilies of proteins responsible for motility. While members of both protein families generate force and are responsible for producing motion, they show no detectable sequence homology, despite the fact that their three-dimensional structures are clearly related. We have determined the homologous regions of the two proteins by structural comparison. This has revealed a conserved structural architecture shared with other ATPases, and has revealed residues conserved between kinesin and myosin. We are currently examining these residues to understand their function. We are also comparing the conserved structural regions to other motors, in particular dynein, and also searching for novel members of this fold family by searching sequence databases.

传统的驱动蛋白和肌球蛋白是两种蛋白的创始成员 负责运动的不同蛋白质超家族。 尽管 两个蛋白质家族的成员都产生力量并负责 为了产生运动，它们没有表现出可检测到的序列同源性， 尽管事实上它们的三维结构是清晰的 有关的。 我们已经确定了两者的同源区域 通过结构比较蛋白质。 这揭示了一个保守的 与其他 ATP 酶共享的结构体系，并揭示了 驱动蛋白和肌球蛋白之间保守的残基。 我们目前 检查这些残基以了解它们的功能。 我们也是 将保守的结构区域与其他电机进行比较， 特别是动力蛋白，并且还在寻找该折叠的新成员 通过搜索序列数据库来确定家族。