NATIONAL RESOURCE FOR ZEBRAFISH

国家斑马鱼资源

• 批准号: 6015812
• 负责人: Monte Westerfield
• 金额: $ 2.86万
• 依托单位: UNIVERSITY OF OREGON
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-05-01 至 2003-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6015812
• 关键词: Internet; animal care; animal colony; animal genetic material tag; animal population genetics; communicable disease transmission; computer system design /evaluation; cryopreservation; developmental genetics; epizootiology; gene mutation; genetic models; genetic registry /resource /referral center; genetic strain; handbook; information systems; mutant; sperm; veterinary medicine; veterinary science; zebrafish

DESCRIPTION (Adapted from the applicant's abstract): Zebrafish have recently become more commonly used animals for studies of vertebrate development and genetics. Powerful techniques allow efficient generation and recovery of zebrafish mutations that affect a wide range of genes, including ones that regulate developmental patterning, organogenesis, physiology, and behavior. The functions of many of these genes appear to be conserved among different vertebrate groups. Thus, analysis of zebrafish mutations provides insights to gene functions in other vertebrates, including humans. Ongoing genetic screens have identified over 7,000 mutations; the fish that carry them are distributed among more than 100 laboratories in 28 countries. The zebrafish research community would benefit from a centralized site to preserve and keep track of these stocks, and to facilitate their distribution to researchers. This application seeks funds to establish a National Resource for Zebrafish to maintain wild-type and mutant stocks of the highest possible quality and uniformity, and to take these stocks readily available to the international research community. The proposed information center will: 1) develop and maintain a computer database, accessible via the Internet; 2) publish a manual for the laboratory use of zebrafish and a periodical with news about zebrafish research and techniques; 3) facilitate communication among zebrafish researchers; and 4) host visits from researchers who wish to work with stocks or learn techniques to identify and maintain mutants. The stock center will: 1) establish standards and procedures for generating healthier and more vigorous strains; 2) characterize diseases endemic to laboratory stocks; 3) development methods for disease control and treatment; and 4) publish a manual of procedures for preventing, diagnosing, and treating zebrafish diseases.

描述（改编自申请人的摘要）：斑马鱼 最近成为脊椎动物研究的常用动物 发展和遗传学。强大的技术允许有效发电 以及影响广泛基因的斑马鱼突变的恢复， 包括调节发育模式，器官发生， 生理和行为。这些基因的许多功能似乎 在不同的脊椎动物组中保守。因此，分析 斑马鱼突变为其他基因功能提供了见解 脊椎动物，包括人类。正在进行的遗传筛选已经确定 超过7,000个突变；携带它们的鱼被分配更多 28个国家 /地区的100个实验室。斑马鱼研究社区 将从集中式站点中受益，以保存并跟踪这些 股票，并促进其分配给研究人员。这 申请寻求资金为斑马鱼建立国家资源 维持最高质量的野生型和突变库存 统一，并将这些股票随时可用 国际研究界。拟议的信息中心将：1） 开发和维护计算机数据库，可通过Internet访问； 2） 出版一本用于实验室使用斑马鱼的手册，并与 有关斑马鱼研究和技术的新闻； 3）促进沟通 斑马鱼研究人员； 4）希望的研究人员的主持人访问 与股票合作或学习识别和维护突变体的技术。 股票中心将：1）建立标准和程序 产生更健康，更剧烈的菌株； 2）表征疾病 实验室股票的地方； 3）疾病控制的发展方法 和治疗； 4）发布一本防止程序手册， 诊断和治疗斑马鱼疾病。