Zebrafish International Resource Center

斑马鱼国际资源中心

• 批准号: 10516402
• 负责人: Monte Westerfield
• 金额: $ 4.31万
• 依托单位: UNIVERSITY OF OREGON
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-01-01 至 2023-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10516402
• 关键词: Adopted; Affect; American; Animals; Antisense Oligonucleotides; Aquaculture; Behavior; Behavioral Genetics; Biological Assay; Communities; Consultations; Development; Doctor of Philosophy; Fishes; Funding; Generations; Genes; Genetic; Genetic Diseases; Goals; Grant; Human; International; Laboratories; Methods; Modeling; Mutation; Organism; Organogenesis; Pathology; Pattern; Physiology; Recovery; Research; Resources; Sampling; Services; Techniques; Technology; Transgenic Organisms; Vertebrates; Yakama Indian; Zebrafish; base; career; diagnostic screening; gene function; insight; member; pathogen; repository; screening services

Overall Project Summary/Abstract Zebrafish is a premiere organism to study vertebrate development, physiology, behavior, genetics and disease. Powerful techniques allow efficient generation and recovery of zebrafish mutations affecting genes that regulate developmental patterning, organogenesis, physiology and behavior. Recent advances make it easy to study gene function in transgenic zebrafish and with antisense oligonucleotides. The functions of many, if not most of these genes are conserved among vertebrate groups. Thus, analysis of zebrafish mutations provides insights into gene function in other vertebrates, including humans. The Zebrafish International Resource Center has been established as a repository that provides animals, materials and services to the research community. This proposal seeks continued funding 1) to serve as a central repository for zebrafish genetic stocks and research materials, 2) to provide consultation and pathology services, and 3) to develop a platform, based on a panel of PCR assays, to screen for the most prevalent pathogens of laboratory zebrafish. These materials, services and information will be made widely available to the research community. Diversity Supplement Summary/Abstract We apply for a diversity supplement to the P40 grant (OD011021) of the Zebrafish International Resource Center (ZIRC) for Mr. Corbin Jaid Schuster, an American Citizen and member of the Yakama Nation. The research plan ties Aims 2 and 3 of the ZIRC P40 grant to Corbin’s long-term career goal to employ the zebrafish model in research and to develop similar wild and aquaculture fish diagnostic screening services for the Yakama Nation. In turn, ZIRC will benefit from adopting the environmental sampling method and dPCR technology, which Corbin developed during his Ph.D.

总体项目总结/摘要 斑马鱼是研究脊椎动物发育、生理学、行为、遗传学和 强大的技术可以有效产生和恢复影响斑马鱼的突变。 调节发育模式、器官发生、生理和行为的基因。 进展使得研究转基因斑马鱼的基因功能和反义变得容易 许多（如果不是大多数）这些基因的功能在脊椎动物中是保守的。 因此，对斑马鱼突变的分析可以深入了解其他脊椎动物的基因功能， 包括人类。 斑马鱼国际资源中心已建立作为一个存储库，提供 该提案寻求持续资助1) 作为斑马鱼遗传资源和研究材料的中央存储库，2) 提供 咨询和病理学服务，以及 3) 开发一个基于 PCR 检测组的平台， 筛查实验室斑马鱼最常见的病原体。 信息将广泛提供给研究界。 多样性补充摘要/摘要 我们申请斑马鱼国际资源 P40 拨款 (OD011021) 的多样性补充 美国公民、亚卡马民族成员 Corbin Jaid Schuster 先生的中心 (ZIRC)。 研究计划将 ZIRC P40 赠款的目标 2 和 3 与 Corbin 的长期职业目标联系起来，即雇用 研究斑马鱼模型并开发类似的野生和水产鱼类诊断筛查服务 反过来，ZIRC 也将受益于采用环境采样方法和 dPCR 技术是 Corbin 在攻读博士学位期间开发的。