INDUCTION OF CERVICAL RIPENING W/ INTERLEUKIN 8 (IL8) IN PREGNANT RHESUS MONKEYS

用白细胞介素 8 (IL8) 诱导怀孕恒河猴的宫颈成熟

• 批准号: 6277370
• 负责人: GEORGE J HALUSKA
• 金额: $ 7.42万
• 依托单位: OREGON REGIONAL PRIMATE RESEARCH CENTER
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-05-01 至 1999-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6277370
• 关键词: Mammalia; Primates; clinical research; endocrine gland /system; infant mortality; low birth weight infant human; mother /infant health care; reproductive system; women's health

Interleukin-8 (IL-8) is a cytokine that induces selective neutrophil chemotaxis and activation. Progesterone inhibits and antiprogestins stimulate production of IL-8 by chorion and decidua. Cervical ripening is associated with an influx of inflammatory cells into the cervix, but the role of IL-8 in primate parturition remains to be established. To investigate the role of IL-8 in cervical ripening, we administered purified, human fibroblast-derived IL-8 intravaginally to 3 pregnant monkeys beginning at 137-139 days of gestation (term = 167 days) at a dose of 150 ng/kg. The cervix was evaluated by a modified Bishop's Score. IL-8 was administered in vehicle (2.5% hydroxypropyl cellulose [HPC] with 300 fl Evans Blue solution) by inserting a 1.0 or 3.0 ml syringe into the vaginal vault. Amniocentesis was performed at intervals to determine changes in amniotic fluid prostaglandins and cytokines. After a recovery period of 4-5 days, a second dose of IL-8 (300 ng/kg) was administered. The effect of graded doses of intravaginal IL-8 on modified Bishop's Scores (mean q SEM) Dose 0 hours 24 hours 48 hours 72 hours 150 ng/kg 0.6 q 0.3 5.6 q 0.3* 5.0 q 1.2* 5.3 q 0.3* 300 ng/kg 2.0 q 1.0 4.0 q 3.0 6.5 q 0.5* * different from time 0 hrs at P<0.05 (t-test). Two out of three animals showed an increase in amniotic fluid IL-8 concentrations but there were no changes in amniotic fluid PGE2 or PGF2`. One animal delivered after the second dose of IL-8. Intravaginal application of purified human fibroblast-derived IL-8 induces significant cervical ripening in late pregnant rhesus monkeys. This data may have important implications in therapies related to induction of cervical ripening and labor.

白介素8（IL-8）是一种诱导选择性的细胞因子 中性粒细胞趋化性和激活。 孕酮抑制和 抗膜蛋白通过绒毛膜和Decidua刺激IL-8的产生。 宫颈成熟与炎症细胞的涌入有关 进入子宫颈，但是IL-8在灵长类动物分娩中的作用仍然存在 要建立。 研究IL-8在宫颈中的作用 成熟，我们管理纯化的人类成纤维细胞衍生的IL-8 从137-139天开始静脉注入3个怀孕的猴子 妊娠（术语= 167天），剂量为150 ng/kg。 子宫颈是 通过修改后的主教分数进行评估。 IL-8在 车辆（2.5％羟丙基纤维素[HPC]，蓝色300 fl Evans Blue 解决方案）通过将1.0或3.0 mL注射器插入阴道库中。 以间隔进行羊膜穿刺术，以确定 羊水前列腺素和细胞因子。 恢复期后 在4-5天内，给予第二剂IL-8（300 ng/kg）。 这 渐变剂量的阴道内IL-8对改良主教的影响 分数（平均Q SEM） 剂量 0小时 24小时 48小时 72小时150 ng/kg 0.6 Q 0.3 5.6 Q 0.3* 5.0 Q 1.2* 5.3 Q 0.3* 300 ng/kg 2.0 Q 1.0 4.0 Q 3.0 6.5 Q 0.5* *与p <0.05（t检验）时0小时不同的时间不同。 三分之二 动物显示羊水IL-8浓度的增加，但 羊水PGE2或PGF2`没有变化。 一只动物 在第二剂IL-8后交付。 腔内应用 纯化的人成纤维细胞衍生的IL-8诱导明显的宫颈 成熟的怀孕恒河猴。 这些数据可能具有 与宫颈诱导有关的疗法的重要意义 成熟和劳动。