ACTH INFUSION INTO RHESUS MONKEY FETUS LEADS TO PREMATURE LABOR & DELIVERY

向恒河猴胎儿注入促肾上腺皮质激素会导致早产

• 批准号: 6592323
• 负责人: GEORGE J HALUSKA
• 金额: $ 11.11万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-05-01 至 2003-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6592323
• 关键词: Mammalia; clinical research; endocrine gland /system; health care; mother /infant health care; reproductive system; women's health

The activation of the hypothalamic-pituitary-adrenal axis in fetal sheep is the primary initiator of parturition; however, this same mechanism has not been proven to be important in primate parturition. To determine if adrenocorticotropic hormone (ACTH) can induce premature parturition in primates, we infused ACTH (0.1 U in 30 min, every 2 hrs; n=7) beginning on day 135 of pregnancy (term=167 days) until delivery occurred. A control group of animals (n=3) received saline vehicle infusions. All animals were surgically instrumented with fetal and maternal vascular and amniotic fluid catheters, fetal ECG and myometrial EMG electrodes. Uterine activity (UA) and fetal ECG were recorded continuously. The results of ACTH and vehicle infusion are listed in the following table (values are mean + SEM) * = P<0.05 (t-test) ACTH vs Vehicle Begin Infusion (day of preg) Onset Nocturnal UA (days) Days to Delivery Delivery (Day of Pregnancy) ACTH (n=6) 135.3 + 0.6 6.3 + 0.8 15.2 + 1.3 149.7 + 1.3 Vehicle (n=3) 135.7 + 1.8 24.7 + 2.7* 27.6 + 3.2* 163.3 + 1.5* One ACTH-treated animal was delivered by cesarean section at 165 days of pregnancy after prolonged labor and dystocia and was excluded from the data set. Mean (+ SEM) maternal and fetal steroid changes in ACTH-treated animals *=P<0.005 (Rank Sum Test) (n=6) Fetal Cortisol (ng/ml) Fetal DHEA-Sulfate (?g/ml) Fetal Androstene-dione (ng/ml) Fetal Estrone (pg/ml) Maternal Estradiol (pg/ml) Baseline 79 + 11 0.4 + .08 0.8 + 0.2 97 + 27 360 + 45 Labor 276 + 48* 6.6 + 3.1* 7.4 + 1.7* 519 + 170* 877 + 187* We conclude that infusion of ACTH into the fetal circulation activates fetal adrenal steroid biosynthesis causing increased secretion of glucocorticoids and androgens leading to increased estrogen and cortisol production and pre-term delivery in rhesus monkeys. FUNDING NIH HD06159, HD18185 PUBLICATIONS None

胎儿下丘脑 - 垂体 - 肾上腺轴的激活 绵羊是分娩的主要发起者；但是，也是如此 在灵长类动物的分娩中，机制尚未被证明很重要。 确定肾上腺皮质激素（ACTH）是否可以诱导 在灵长类动物中过早分娩，我们注入了ACTH（30分钟内0.1 U， 每2个小时； n = 7）从怀孕的第135天开始（期限= 167天） 直到交货发生。 接收的动物对照组（n = 3） 盐水输注。 所有动物均已手术仪器 胎儿和母体血管和羊水导管，胎儿 ECG和肌层EMG电极。 子宫活性（UA）和胎儿 ECG连续记录。 ACTH和车辆的结果 下表列出了输液（值为平均 + SEM） * = p <0.05（t检验）ACTH与车辆开始输注（Preg Day）发作 夜间UA（天数）交货天数（怀孕日）ACTH （n = 6）135.3 + 0.6 6.3 + 0.8 15.2 + 1.3 149.7 + 1.3车辆（n = 3）135.7 + 1.8 24.7 + 2.7* 27.6 + 3.2* 163.3 + 1.5*一只经ACTH处理的动物是 长时间后，由剖宫产部分在怀孕的165天后交付 劳动力和障碍症，被排除在数据集之外。 平均（+ SEM） ACTH处理的动物的母体和胎儿类固醇的变化 *= P <0.005 （等级总测试）（n = 6）胎儿皮质醇（Ng/ml）胎儿DHEA-硫酸盐 （？g/ml）胎儿雄激素 - 二酮（ng/ml）胎儿雌酮（pg/ml）母体 雌二醇（PG/mL）基线79 + 11 0.4 + .08 0.8 + 0.2 97 + 27 360 + 45劳动276 + 48* 6.6 + 3.1* 7.4 + 1.7* 519 + 170* 877 + 187*我们 得出结论，将ACTH输注到胎儿循环中会激活 胎儿肾上腺类固醇生物合成，导致分泌增加 糖皮质激素和雄激素导致雌激素增加和 恒河猴的皮质醇生产和术前分娩。 资金 NIH HD06159，HD18185出版物无