TOPICAL MICROBICIDES FOR PREVENTION OF STD & HIV INFECTION

预防性病的外用杀菌剂

• 批准号: 6116381
• 负责人: DOROTHY L PATTON
• 金额: $ 8.67万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-05-01 至 2000-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6116381
• 关键词: AIDS; Mammalia; Primates; bacteria; biological products; clinical research; immunology; infection; inflammation; lymphatic system; reproductive system; sexually transmitted diseases; virus; women's health; AIDS; Mammalia; Primates; bacteria; biological products; clinical research; immunology; infection; inflammation; lymphatic system; reproductive system; sexually transmitted diseases; virus; women's health

The potential effectiveness of a boric acid gel and a 0.25% chlorhexidine gluconate (CHG) gel as vaginal microbicides were assessed. The boric acid gel was compared with a placebo gel (containing no boric acid) for effects on the vaginal ecosystem. After the monkeys received a single application of either gel, no deleterious effects were noted by colposcopy during a one-week follow up. Monkeys receiving boric acid showed an increase in lactobacilli, a beneficial microorganism in the vaginal ecosystem. Levels remained elevated for two days. No other significant microbiological changes were noted after application of either gel. Then the anti-chlamydial effects of 0.25% CHG were assessed in this model. A single application of 0.25% CHG was followed by an intravaginal inoculation with Chlamydia trachomatis in a group of monkeys, while positive control animals were inoculated with Chlamydia without pretreatment with CHG. Animals were examined by modified colposcopy, and cervical and vaginal swabs were collected for detection of Chlamydia and vaginal microflora for two weeks after inoculation. Evidence of chlamydial infection was not detected in any of the animals pretreated with 0.25% CHG, whereas all positive controls were infected. Changes in vaginal flora were minimal and transient in all animals. Colposcopic observations also showed minimal and transient changes to the vaginal epithelium in a small subset of animals.

评估了硼酸凝胶和0.25％葡萄糖酸（CHG）凝胶作为阴道菌心的潜在有效性。 将硼酸凝胶与安慰剂凝胶（无硼酸）进行比较，以对阴道生态系统作用。 在猴子收到任何一种凝胶的应用后，在为期一周的随访中，阴道镜检查没有任何有害影响。 接受硼酸的猴子表现出乳酸杆菌的增加，这是阴道生态系统中有益的微生物。 水平保持升高两天。 两种凝胶施用后，均未注意到其他显着的微生物变化。 然后在该模型中评估了0.25％CHG的抗粉刺作用。 在一组猴子中，单次施用0.25％的CHG之后，在一组猴子中与沙眼衣原体接种了阴道内接种，而阳性对照动物则与衣原体接种而没有用CHG进行预处理。 通过修饰的阴道镜检查动物，并收集宫颈和阴道拭子以检测衣原体和阴道菌群后两周。 在用0.25％CHG预处理的任何动物中均未检测到衣原体感染的证据，而所有阳性对照均被感染。 所有动物中阴道菌群的变化均极小且短暂。 阴道镜观察还显示出一小部分动物中阴道上皮的变化最小和短暂的变化。