IN VIVO EVALUATION OF PRIMATE LENTIVIRUSES

灵长类慢病毒的体内评估

• 批准号: 6116341
• 负责人: William Randall Morton
• 金额: $ 8.67万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-05-01 至 2000-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6116341
• 关键词: AIDS; Mammalia; Primates; immunology; infection; inflammation; lymphatic system; model design /development; sexually transmitted diseases; virus

HIV-2287 is a viral isolate derived from in vivo passage 2 of HIV-2EHO in Macaca nemestrina. Part of this experiment was to evaluate the pathogenic potential of this virus in other macaque species. Two M. mulatta and two M. fascicularis were inoculated with 5,000 TCID intravenously. The two M. mulatta became infected at very low levels and did not show any depletion of the CD4+ cell population. However, the two M. fascicularis became infected at higher levels in the peripheral blood, and both showed dramatic loss of the CD4+ cell population, one becoming virtually depleted within 4 weeks post-inoculation. This species variation warrants more investigation. Another part of this project is evaluation of the infectivity and pathogenesis of other lentivirus isolates. A lymph node had been isolated from another macaque that was part of the in vivo passage of HIV-2EHO (passage 3). A viral isolate recovered from this lymph node was inoculated into one M. nemestrina as part of this project. This isolate produced infection and CD4+ cell depletion in a time frame comparable to the HIV-2287. This may expand the available pathogenic isolates for future research.

HIV-2287是一种源自Macaca Nemestrina的HIV-2EHO的体内体体2的病毒分离株。 该实验的一部分是评估该病毒在其他猕猴物种中的致病潜力。 静脉注射5,000个TCID接种了两个M. mulatta和两个筋膜分枝杆菌。 两个M. Mulatta被感染的水平非常低，没有显示CD4+细胞群的任何耗竭。 然而，两种筋膜分枝杆菌在外周血中被较高的水平感染，并且两者都显示出CD4+细胞群的巨大损失，在接种后4周内，一个人几乎耗尽了。 该物种变化值得进行更多的研究。该项目的另一部分是评估其他慢病毒分离株的感染性和发病机理。 已经从另一个猕猴中分离出淋巴结，该猕猴是HIV-2EHO体内通过的一部分（通道3）。 从该淋巴结中回收的病毒分离株作为该项目的一部分接种到一个Nemestrina中。 该分离物在与HIV-2287相当的时间范围内产生的感染和CD4+细胞耗竭。 这可能会扩大可用的致病株以供将来的研究。