Core--Xiphophorus Genetic Stock Center

核心--剑尾鱼种质中心

• 批准号: 6259639
• 负责人: STUART W CALHOUN
• 金额: $ 27.06万
• 依托单位: TEXAS STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-05-01 至 2004-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6259639
• 关键词: Osteichthyes; alternatives to animals in research; animal breeding; animal colony; animal genetic material tag; biomedical facility; genetic models; genetic registry /resource /referral center

Description: (Applicant's Description) The Xiphophorus Genetic Stock Center at Southwest Texas State University will serve as the core facility for the Program Project because (1) all stocks necessary for the production of hybrid and backcross hybrid fishes required in the Program Project are maintained as pedigreed lines at the Stock Center, (2) the Stock Center is the only facility within the two research institutions that has the capacity to house and feed the estimated 2,000 animals that will be bred per year during the course of the estimated 2,000 animals that will be bred per year during the course of the project, and (3) proposed experimental treatments with UV and MNU occur early in the life of the backcross hybrids, and since experimental animals must be maintained for six months or more prior to sacrifice, the Stock Center is the most logical facility for headquartering the core. The specific aims of the Core Project are to ensure that (1) parental stocks required to produce hybrids are maintained in sufficient numbers, (2) all hybridizations produce sufficient F1 progeny for backcross matings, (3) backcross offspring are produced in sufficient numbers for the experimental and control of requirements produced in sufficient numbers for the experimental and control requirements of each project (4) backcross offspring receive the precise experimental treatment, i.e., UV irradiation or MNU dosage specified by each project investigator, (5) the health of the entire animal colony is never compromised, and (6) the genetic backgrounds and stock purity of pedigrees used in these experiments are well characterized. Baseline data regarding the genetic backgrounds of parental and F1 hybrid stocks used to manufacture backcross hybrids are necessary, particularly in light of recent findings of polyallelism in isogenic clonal zebrafish stock which suggest that assumptions of stock homogeneity must also be validated in Xiphophorus stocks. Biochemical genetic profiles for each of the stocks will identify candidates for additional hybridization mating required by each component project to continue their linkage map saturation and/or associated tumor marker mapping efforts. Core facility personnel will screen parents of all broods utilized in the Program for more than 50 protein-coding loci after each mating has been terminated and will screen siblings of the animals used in the Program Project. The significance of establishing a core facility for the Program Project is that it will centralize stock management, parental, hybrid, and backcross animal product, experimental treatment, animal core, and stock purity, thereby streamlining the logistics of the Program Project and substantially decreasing the cost of research.

描述：（申请人的描述）西南德克萨斯州立大学的剑尾鱼遗传资源中心将作为该计划项目的核心设施，因为（1）该计划项目所需的杂交和回交杂交鱼类生产所需的所有资源均保持为种畜中心的纯种品系，(2) 种畜中心是两个研究机构内唯一有能力饲养和饲养大约 2,000 只动物的设施，这些动物将在研究期间每年繁殖。估计在该项目期间每年将繁殖 2,000 只动物，并且 (3) 提议的 UV 和 MNU 实验处理发生在回交杂交种生命的早期，并且因为实验动物必须在之前保存六个月或更长时间就牺牲而言，库存中心是最合理的核心总部设施。核心项目的具体目标是确保 (1) 维持生产杂交所需的亲本种群数量充足，(2) 所有杂交都能产生足够数量的 F1 后代用于回交交配，(3) 产生足够数量的回交后代实验和控制要求 产生足够数量以满足每个项目的实验和控制要求 (4) 回交后代接受精确的实验处理，即每个项目研究者指定的紫外线照射或 MNU 剂量， (5) 回交后代的健康状况整个动物群落从未受到损害，并且（6）这些实验中使用的谱系的遗传背景和种群纯度得到了很好的表征。有关用于制造回交杂交体的亲本和 F1 杂交种群遗传背景的基线数据是必要的，特别是考虑到最近在同基因克隆斑马鱼种群中发现的多等位性，这表明种群同质性的假设也必须在剑尾鱼种群中得到验证。每个种群的生化遗传图谱将识别每个组成项目所需的额外杂交交配的候选者，以继续其连锁图谱饱和和/或相关肿瘤标记物作图工作。每次交配结束后，核心设施工作人员将筛查该计划中使用的所有幼崽的父母，以获取 50 多个蛋白质编码位点，并将筛查该计划项目中使用的动物的兄弟姐妹。为该计划项目建立核心设施的意义在于，它将集中库存管理、亲本、杂交和回交动物产品、实验处理、动物核心和库存纯度，从而简化该计划项目的物流并大幅减少研究成本。