EPIZOOTIOLOGY OF MACHUPO VIRUS IN BOLIVIA

玻利维亚马丘波病毒的流行病学

• 批准号: 6201308
• 负责人: Clarence J. Peters
• 金额: $ 12.49万
• 依托单位: UNIVERSITY OF NEW MEXICO
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-08-01 至 2001-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6201308
• 关键词: Arenaviridae; Rodentias; South America; disease reservoirs; disease vectors; epizootiology; nucleic acid sequence; polymerase chain reaction; serology /serodiagnosis

Machupo virus (MV; Arenaviridae: Tacaribe VComplex; Machupo) is the etiologic agent for Bolivian Hemorrhagic Fever (BHF). MV is a rodent- borne, segmented, negative-sense RNA virus that shares many replicative, pathophysiologic, and epidemiologic features with the genus Hantavirus, family Bunyaviridae. As with hantaviruses, each arenavirus species except one is harbored by a specific rodent host in the family Muridae. Less is known about the specifics and geographic feature of these viral/host associations than for those of the hantaviruses and their hosts. This project will evaluate the relationship between prevalence of Machupo virus infection in wild populations of Bolivian rodents in relation to human land use patterns and rodent ecology. Using previously collected specimens, we will examine rodents of the murid subfamily Sigmodontinae from throughout Bolivia for seroprevalence of Machupo virus antibodies and evaluate any variation in levels of infection in light of differences in rodent abundance and difference in habitat. Viruses and rodent reservoir species will be characterized using cytogenetic and molecular genetic techniques. Specifically, we propose to test the following hypotheses; 1) The distribution of human cases of BHF is a function of habitat type and not geographic distribution of the reservoir species, 2) Human land-use patterns including habitat disturbance in northern Bolivia favors increased abundance of MV reservoir species and thus increases the risk of human disease the risk of human disease, and 3) levels of rodent abundance are positively correlated with seroprevalence of MV (e.g., as rodent populations increase in density, the percentage of the population infected with MV increases). These data will greatly expand our knowledge of MV and our understanding of Arenaviruses in general.

Machupo病毒（MV； Arenaviridae：Tacaribe Vcomplex; Machupo）是 玻利维亚出血热（BHF）的病因学剂。 MV是啮齿动物 - 传播，分割，负敏感的RNA病毒具有许多复制性， 汉坦病毒属的病理生理和流行病学特征， 家庭Bunyaviridae。 与汉坦病毒一样，除 一个由穆里达家族中的特定啮齿动物主持人藏有一个。 少是 知道这些病毒/宿主的细节和地理特征 关联比汉坦病毒及其宿主的关联。 该项目将评估Machupo的患病率 玻利维亚啮齿动物野生种群的病毒感染与 人类土地使用模式和啮齿动物的生态学。 使用先前收集的 标本，我们将检查Murid亚家族Sigmodontinae的啮齿动物 从玻利维亚到整个玻利维亚的血清阳性病毒抗体的血清阳性和 根据差异的差异，评估感染水平的任何变化 啮齿动物的丰度和栖息地的差异。 病毒和啮齿动物水库 物种将使用细胞遗传学和分子遗传来表征 技术。 具体而言，我们建议检验以下假设。 1） BHF案例的分布是栖息地类型的函数， 不是储层物种的地理分布，2）人类土地利用 包括北玻利维亚北部栖息地干扰在内的模式增加了 丰富的MV储层物种，因此增加了人类的风险 疾病人类疾病的风险和3）啮齿动物丰度的水平是 与MV的血清阳性正相关（例如，作为啮齿动物 人群的密度增加，被感染的人口百分比 随着MV的增加）。 这些数据将大大扩展我们对MV和 我们对竞技病毒的理解总体上。