MESOCORTICAL ANATOMY AND PLASTICITY

中皮质解剖学和可塑性

• 批准号: 2839275
• 负责人: GARY W VAN HOESEN
• 金额: $ 23.99万
• 依托单位: UNIVERSITY OF IOWA
• 依托单位国家: 美国
• 财政年份: 1979
• 资助国家: 美国
• 起止时间: 1979-01-01 至 2000-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2839275
• 关键词: Alzheimer's disease; Primates; cerebral cortex; confocal scanning microscopy; entorhinal cortex; fluorescent dye /probe; histochemistry /cytochemistry; human tissue; immunocytochemistry; limbic system; neuroanatomy; neurons; phenotype

This continuation (years 17-20) describes experiments designed to investigate the structural organization of the mesocortices of the limbic lobe in the monkey and human brain. In its initial years, reinnervation of the amygdala was investigated and the term "plasticity" in the title has continued with the grant, although it is not longer descriptive of the research proposed. A central goal has been to investigate cortical association axons that link the association areas with the mesocortices. The reciprocal of this relationship has been investigated as well. In 1989 Alzheimer's disease research was added as a new direction since it targets the exact neurons that mediate this relationship. In the grant's last competitive review dual efforts relating to Alzheimer's disease research and nonhuman primate experimental neuroanatomical research were proposed, and the grant received a Javits Neuroscience Investigator Award. In this proposal, three new aims are outlined. Two deal with combined human and monkey investigations and aim to analyze Brodmann's areas 35 and 34. The former interconnects the entorhinal mesocortices with the temporal isocortices. Area 35 is understood poorly in the human brain and the first to develop neurofibrillary tangles in Alzheimer's disease. Monkey behavioral studies link it closely to associative memory. Area 34 forms the anterior and medial entorhinal cortex and contains laminar alterations in some brains of schizophrenics. It is damaged directly in all types of partial and traumatic herniation of medial temporal lobe through the incisura of the tentorium cerebelli. Cellular phenotypes and boundaries will be established for these areas, focusing both on projection and intrinsic neurons. An additional aim utilizes only the monkey and will assess "three neuron" neural systems related to the mesocortices. Specifically, the terminal fields of entorhinal neurons will be injected with a retrograde tracer and the labeled neurons will be filled intracellularly with Lucifer yellow to reveal their morphology. In the same preparation afferent axons projecting to characterized entorhinal neurons will be anterogradely labeled to establish their putative input patterns to the filled neuron. Confocal laser scanning microscopy will be used to analyze these preparations. For all aims, the data will provide baseline neuroscience information for cortical areas known to be involved in developmental, neurological and psychiatric disease. It is cost effective research that draws on a collection of non-human primate and human material gathered over the past 27 years at Harvard Medical School (1969-1978) and The University of Iowa (1979-present).

这种延续（17 - 20年）描述了旨在的实验 研究边缘的中皮皮质的结构组织 猴子和人脑中的叶。 在最初的几年，加剧了 调查了杏仁核和标题中的“可塑性”一词 尽管不再描述 研究提出了。 一个中心目标是调查皮质 将关联区域与中皮层联系起来的关联轴突。 也已经研究了这种关系的倒数。 在 1989年，阿尔茨海默氏病研究被添加为新方向 它针对介导这种关系的确切神经元。 在 格兰特的最后一次竞争评论与阿尔茨海默氏症有关的双重努力 疾病研究和非人类灵长类动物实验性神经解剖学 提出了研究，并获得了Javits神经科学 研究者奖。 在此提案中，概述了三个新目标。 两项与人类和猴子的调查交易，旨在 分析布罗德曼的区域35和34。前者互连 与颞叶等皮质的内hin骨中质皮质。 区域35是 在人的大脑中了解得很糟糕，也是第一个发展的 阿尔茨海默氏病的神经纤维缠结。 猴子行为 研究将其紧密地链接到关联记忆。 区域34形成 前和内侧内部皮层，并包含层层层次的改变 在精神分裂症患者的一些大脑中。 它直接在所有类型中损坏 临时颞叶的局部和创伤性疝 小脑tentorium的incisura。 细胞表型和 这些领域将建立界限，重点放在 投影和内在神经元。 另一个目标仅利用 猴子并将评估与 中皮皮。 具体而言，内嗅神经元的末端场 将注射逆行示踪剂，标记的神经元将 细胞内充满路西法黄色，以揭示他们的 形态学。 在相同的准备工作中，轴突投射到 特征性内嗅神经元将被同类标记为 将其推定的输入模式建立到填充的神经元。 共焦 激光扫描显微镜将用于分析这些制剂。 对于所有目的，数据将提供基线神经科学信息 对于已知参与发育，神经系统的皮质区域 和精神病。 这是经济有效的研究，借鉴了 收集非人类灵长类动物和人类物质的收集 过去27年在哈佛医学院（1969-1978）和 爱荷华大学（1979年至今）。