DEVELOPMENTAL REGULATION IN DROSPHILLA

果蝇的发育调控

• 批准号: 2900690
• 负责人: FRANK LASKI
• 金额: $ 19.16万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-07-01 至 2002-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2900690
• 关键词: DNA binding protein; Drosophilidae; alleles; chromosome deletion; developmental genetics; electron microscopy; gene expression; gene interaction; genetic translation; histochemistry /cytochemistry; histogenesis; leg; light microscopy; microinjections; nucleic acid sequence; oogenesis; protein binding; protein structure function; transcription factor; transposon /insertion element

The gene bric a brac (bab) is a transcriptional regulator that is required for the proper development of the ovary, leg and antenna in Drosophila melanogaster. Our long term goal is to have a molecular understanding of how bab functions, and to use bab as an entryway to the investigation of limb and ovary development. We propose to continue our studies by means of the following specific aims: 1. A functional analysis of bab. We have extensive knowledge of the gene structure, genetics and expression pattern of bab. We have identified a target gene for bab (it binds its own locus) and shown that bab can repress its own production. Our current goal is to understand how bab functions at a molecular level. To this we will investigate the consequence of babII protein binding to a promoter. In addition, we will do structure/function analysis of the babII protein, investigating the function of the BTB domain and psq motif. 2. A screen for genes that interact with bab during leg development. bab provides a unique tool in understanding the process of pattern formation in the leg of Drosophila, with specific interest in the formation of the proximal-distal axis. bab is required to give the three central segments of the tarsus their identity, therefore the gene must be involved in the process of interpreting positional cues along the axis. Understanding how bab interacts with other genes to interpret positional information will give insight into the molecular mechanism of proximal- distal axis formation. Using a genetic approach, we will identify genes that interact with bab. These genes will be studied to determine their role in leg development. 3. A genetic and molecular characterization of the ntf (no terminal filaments) gene. We have recently identified that ntf gene, the second gene identified (in addition to bab) that is required for terminal filament formation. Our long term goal is to understand how developmental processes work during ovary development. We are taking a genetic approach to this goal, identifying genes that are required for ovary development and then analyzing these genes. Analysis of bab has told us a great deal about ovary development, the molecular and genetic characterization of the ntf gene should be just as informative.

基因 bric a brac (bab) 是一个转录调节因子 卵巢、腿和触角正常发育所需的 黑腹果蝇。 我们的长期目标是拥有一个分子 了解 bab 的功能，并使用 bab 作为通向 bab 的入口 肢体和卵巢发育的调查。我们建议继续我们的 通过以下具体目标进行研究： 1.bab的功能分析。 我们拥有广泛的知识 bab 的基因结构、遗传学和表达模式。 我们有 确定了 bab 的目标基因（它结合自己的基因座）并表明 bab可以抑制自己的生产。 我们当前的目标是了解 bab 如何在分子水平上发挥作用。 对此我们将调查 babII 蛋白与启动子结合的结果。 此外，我们 将进行 babII 蛋白的结构/功能分析，调查 BTB 结构域和 psq 基序的功能。 2. 筛选在腿部发育过程中与 bab 相互作用的基因。巴布 提供了理解模式形成过程的独特工具 在果蝇的腿上，特别感兴趣的是 近端-远端轴。 bab需要给三个中心 跗节的片段有其身份，因此该基因必须是 参与解释沿轴的位置线索的过程。 了解 bab 如何与其他基因相互作用来解释位置 信息将深入了解近端的分子机制 远端轴的形成。 使用遗传方法，我们将识别基因 与 bab 互动。 将研究这些基因以确定它们的 对腿部发育的作用。 3. ntf 的遗传和分子特征（无末端 丝）基因。 我们最近发现了 ntf 基因，第二个 鉴定出终端所需的基因（除了 bab 之外） 细丝形成。 我们的长期目标是了解如何 发育过程在卵巢发育过程中发挥作用。 我们正在采取 实现这一目标的遗传方法，识别所需的基因 卵巢发育，然后分析这些基因。 分析bab有 告诉我们很多关于卵巢发育、分子和遗传的知识 ntf 基因的表征也应该提供同样丰富的信息。