CENTRAL NEUROCHEMISTRY SERVICE

中央神经化学服务

• 批准号: 4696434
• 负责人: W Z POTTER
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF MENTAL HEALTH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/4696434
• 关键词: 3 methoxy 4 hydroxyphenylethyleneglycol; blood chemistry; cerebrospinal fluid; clinical chemistry; dopamine; epinephrine; gas chromatography mass spectrometry; high performance liquid chromatography; homovanillate; human tissue; hydroxyindoleacetate; neurochemistry; neurotransmitter metabolism; norepinephrine; normetanephrine; serotonin; urinalysis

A joint laboratory effort has begun to provide development of new measures of neurotransmitters and their metabolites as well as more efficient delivery of previously established assays. The bulk of cerebrospinal fluid, plasma and urine assays within the intramural program of the NIMH are analyzed in this facility. Over 5000 samples were analyzed during the last year. Routine analyses include norepinephrine in all tissues, MHPG in CSF, plasma and urine, homovanillic acid and 5HIAA in CSF and plasma, and VMA in urine. Most of these assays can be performed by high performance liquid chromatography (HPLC) although urinary MHPG and VMA still require combined gas chromatography-mass spectrometry. New HPLC assays which have been developed for serotonin, dopamine, epinephrine, and normetanephrine in CSF, epinephrine and DOPAC in plasma and 5HIAA in urine. A GC-MS assay has been developed for normetanephrine. These new assays provide the means of studying interactions between neurotransmitter systems in man as well as distinguishing between differences in metabolism vs turnover.

联合实验室已开始开发新措施 神经递质及其代谢物以及更有效 提供先前建立的测定。 脑脊液的大部分 NIMH 壁内计划内的液体、血浆和尿液检测 在此设施中进行分析。 期间分析了 5000 多个样本 去年。 常规分析包括所有组织中的去甲肾上腺素、所有组织中的 MHPG 脑脊液、血浆和尿液、脑脊液和血浆中的高香草酸和 5HIAA，以及 尿液中的 VMA。 大多数这些测定可以通过高性能进行 尽管尿液 MHPG 和 VMA 仍需要液相色谱 (HPLC) 气相色谱-质谱联用。 新的 HPLC 检测方法 已开发用于血清素、多巴胺、肾上腺素和去甲肾上腺素 血浆中的脑脊液、肾上腺素和 DOPAC 以及尿液中的 5HIAA。 GC-MS 检测具有 被开发用于去甲变肾上腺素。 这些新的检测方法提供了 研究人类神经递质系统之间的相互作用 区分新陈代谢与营业额的差异。