CENTRAL NEUROCHEMISTRY SERVICE

中央神经化学服务

• 批准号: 3845209
• 负责人: W Z POTTER
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF MENTAL HEALTH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3845209
• 关键词: 3 methoxy 4 hydroxyphenylethyleneglycol; 3,4 dihydroxyphenylacetate; biomedical facility; blood chemistry; brain metabolism; cerebrospinal fluid; clinical chemistry; cyclic AMP; dopamine; epinephrine; high performance liquid chromatography; homovanillate; human subject; hydroxyindoleacetate; microdialysis; norepinephrine; normetanephrine; serotonin; tyramine; urinalysis

The central neurochemistry service has provided routine analysis for MHPG, HVA, 5HIAA, 5HT, NE and normetanephrine in plasma. These compounds as well as VMA, DOPAC, epinephrine, dopamine, metanephrine, and 3- methoxytyramine can be measured in urine. In addition, CSF measures of epinephrine, NE, DA, HVA, 5HIAA, MHPG, free and conjugated metanephrine and normetanephrine, and 5HT are possible. Both HPLC with electrochemical detection and GCMS are utilized for quantification. Cumulatively, over 5,000 analyses were provided during the last twelve months. Further, development of microdialysis methods to interpret what contributes to the overall "extracellular" concentrations in brain of a variety of substances including cyclic AMP derives from application of a theoretical model developed in the Biomedical Engineering Program of the National Center for Research Resources. Diffusion profiles around the probe are being determined for major solutes of interest so that the area which microdialysis samples can be accurately specified. This advance is essential to regional brain studies with in vivo microdialysis.

中枢神经化学服务提供了 MHPG 的常规分析， 血浆中的 HVA、5HIAA、5HT、NE 和去甲变肾上腺素。 这些化合物如 以及 VMA、DOPAC、肾上腺素、多巴胺、变肾上腺素和 3- 可以在尿液中测量甲氧基酪胺。 此外，CSF 措施 肾上腺素、NE、DA、HVA、5HIAA、MHPG、游离和结合的变肾上腺素 去甲变肾上腺素和 5HT 是可能的。 HPLC 和电化学 利用检测和 GCMS 进行定量。 累计超过 过去 12 个月内提供了 5,000 项分析。 此外，开发微透析方法来解释什么 有助于大脑中总体“细胞外”浓度 包括环AMP在内的多种物质源自应用 生物医学工程项目中开发的理论模型 国家研究资源中心。 周围的扩散剖面 正在确定感兴趣的主要溶质的探针，以便该区域 可以准确指定哪些微透析样品。 这个预付款是 对于体内微透析的区域脑研究至关重要。